Document 0744
 DOCN  M94A0744
 TI    Optimisation of microculture methods for quantitation of HIV from plasma
       and cellular fractions of peripheral blood.
 DT    9412
 AU    Dunne A; Crowe S; Macfarlane Burnet Centre for Medical Research,
       Fairfield; Hospital, Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:100 (poster no. 50).
       Unique Identifier : AIDSLINE ASHM5/94348911
 AB    OBJECTIVE: Quantitation of HIV in plasma and PBMCs is used in clinical
       trials to follow disease progression and determine drug efficacy. We
       have modified the ACTG protocols to reduce cost and optimise virus
       isolation. METHODS: Microculture procedures as described by the AIDS
       Clinical Trials Group (ACTG) have been used as the basis for these
       procedures. To date we have compared concentrations of PHA and IL-2 used
       for stimulation of donor cells, number of days of stimulation of donor
       cells, use of donor cells from single or multiple donors, and methods of
       isolation and storage of cells and plasma from HIV-infected individuals.
       RESULTS: compared to the ACTG procedures, our preliminary data suggest
       that it is feasible to use stimulation medium for donor cells that has
       no IL-2 and less foetal calf serum, donor cells need only to be
       stimulated for as little as 30 hours before use in HIV culture, a less
       expensive freezing medium for storage of HIV-infected cells provides
       better recovery, and that manipulations of microcultures can be reduced
       to a minimum with no effect on the outcome of the assay. CONCLUSIONS:
       The ACTG protocol can be modified to be more cost effective and less
       time consuming.
 DE    Cost-Benefit Analysis  Human  HIV/*ISOLATION & PURIF  HIV
       Infections/*MICROBIOLOGY  Viremia/*MICROBIOLOGY  *Virus
       Cultivation/ECONOMICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).