Document 0986
 DOCN  M94A0986
 TI    Chlorine dioxide sterilization of red blood cells for transfusion,
       additional studies.
 DT    9412
 AU    Rubinstein A; Chanh T; Rubinstein DB; U.S.C. School of Medicine, Los
       Angeles.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):235 (abstract no. PB0953). Unique
       Identifier : AIDSLINE ICA10/94371589
 AB    The use of sodium chlorite + lactic acid to form the oxidizing agent
       chloride dioxide (ClO2) and chlorous acid has been used as a laboratory
       sterilant for decades using a stock solution (Alcide LD) (15% lactic
       acid and approximately 2.8% sodium chlorite) at dilutions of 1:100 and
       1:150 using 5% dextrose as diluent and have exposed aliquots of washed
       RBC's spiked with HIV-1 for approximately 5 minutes to the sterilant; no
       detectible HIV-1 was assayed at 1:100; at 1:150 there was significant
       but incomplete inactivation. The RBC reducing enzyme system of
       cytochrome b5 and other systems can convert the oxidized Hb
       (methemoglobin) back to Hb. Current experiments using shorter exposure
       times and different dilutions are planned.
 DE    Chlorine/*PHARMACOLOGY  Disinfectants/*PHARMACOLOGY
       Disinfection/*METHODS  *Erythrocyte Transfusion  Erythrocytes/DRUG
       EFFECTS/*MICROBIOLOGY  Human  HIV-1/*DRUG EFFECTS  In Vitro
       Oxides/*PHARMACOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).