Document 1009
 DOCN  M94A1009
 TI    Genetic characterization of HIV-1/2 isolates from Ghana.
 DT    9412
 AU    Osei-Kwasi M; Takehisa J; Ayisi NK; Brandful J; Ampofo W; Magnusen A;
       Hishida O; Igarashi T; Yamashita M; Ido E; et al; Noguchi Memorial
       Institute for Medical Research, University of; Ghana.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):23 (abstract no. 383A). Unique
       Identifier : AIDSLINE ICA10/94371566
 AB    OBJECTIVE: To clarify the phylogenetic relationship of HIV in Ghana, 6
       HIV-1 and 5 HIV-2 isolates from ARC or AIDS patients in 1986, 1990 and
       1992 were sequenced and analyzed. METHODS: A part of pol region suitable
       for comparison among HIV/SIV groups and the env region of HIV-1 were
       sequenced and analyzed. RESULTS: HIV-1 is classified into five major
       subtypes from A to E. Four Ghanaian HIV-1 strains belonged to subtype A,
       one strain to subtype D, and the other one strain remained outside of
       the current classification. Characteristic tetrameric sequences at the
       tip of V3 loop, GLGH, GPGH and GTGR are observed in Ghanaian isolates,
       which have not yet been reported before. Potential N-linked
       glycosylation site is generally well conserved, but Ghanaian strains
       lack two glycosylation sites. In HIV-2, one strain belonged to HIV-2b
       that is a subgroup different from the prototype HIV-2. The topology
       indicates that HIV-2b diverged at the almost same time of divergence of
       SIVsm/SIVmac and other HIV-2 strains reported previously. DISCUSSION AND
       CONCLUSIONS: In Ghana, there is unclassified HIV-1 as well as HIV-1
       subtype A and D. Some of them have V3 tetrameric sequence different from
       those reported previously. Existence of HIV-2b as well as prototype
       HIV-2 was also confirmed.
 DE    Acquired Immunodeficiency Syndrome/*MICROBIOLOGY  Amino Acid Sequence
       AIDS-Related Complex/*MICROBIOLOGY  Comparative Study  *Genes, env
       *Genes, pol  Ghana  Glycosylation  Human  HIV Envelope Protein
       gp120/GENETICS  HIV-1/CLASSIFICATION/*GENETICS/ISOLATION & PURIF
       HIV-2/CLASSIFICATION/*GENETICS/ISOLATION & PURIF  Molecular Sequence
       Data  Peptide Fragments/GENETICS  Variation (Genetics)  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).