Document 1010
 DOCN  M94A1010
 TI    Effect of different variables on the results of microbroth dilution
       method for in vitro antifungal susceptibility testing of yeasts.
 DT    9412
 AU    Maurizio DP; Arzeni D; Barchiesi F; Marinucci G; Ancarani F; Scalise G;
       Institute of Infectious Diseases and Public Health, University of;
       Ancona, Italy.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):23 (abstract no. 385B). Unique
       Identifier : AIDSLINE ICA10/94371565
 AB    OBJECTIVE: We analyzed the effects of various assay conditions on the
       activity of fluconazole, ketoconazole, itraconazole, amphotericin B, and
       flucytosine against 30 strains of Candida spp. and 10 strains each of
       Torulopsis glabrata and Cryptococcus neoformans, isolated from AIDS
       patients, by a microbroth dilution technique. METHODS: The variable
       parameters included: inoculum size (1,000 vs 1,000,000 CFU/ml), medium
       and buffer (RPMI 1640 buffered with MOPS vs BYNB buffered with sodium
       phosphate, both at pH 7), and length of incubation (24 vs 48 hr for
       Candida spp., and T. glabrata, 48 vs 72 hr for C. neoformans). The
       microplates were incubated at 35 degrees C and agitated before reading.
       End-points were determined according to the NCCLS 0 to 4+ criteria for
       the macrobroth dilution method. RESULTS: Increasing the inoculum from
       1,000 to 1,000,000 yeasts cells/ml raised the MICs for all azoles and
       flucytosine against all strains tested. Furthermore, the low inoculum
       permitted the separation between azole-susceptible and -resistant
       strains. Amphotericin B showed the best overall agreement with all time
       readings and the low inoculum sizes. The best agreement between the
       media was found with the low inoculum at the second time reading.
       CONCLUSION: We suggest that the proposed NCCLS parameters for the
       macrobroth assay also appears to produce the greatest agreement in this
       microbroth method.
 DE    Amphotericin B/PHARMACOLOGY  Antifungal Agents/*PHARMACOLOGY
       Candida/*DRUG EFFECTS/GROWTH & DEVELOPMENT  Comparative Study
       Cryptococcus neoformans/*DRUG EFFECTS/GROWTH & DEVELOPMENT  Culture
       Media  Drug Resistance, Microbial  Flucytosine/PHARMACOLOGY  Microbial
       Sensitivity Tests/*METHODS  Temperature  Triazoles/PHARMACOLOGY  MEETING
       ABSTRACT

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