Document 1052
 DOCN  M94A1052
 TI    The GPGQ structure in the V3 loop domain in a Japanese hemophiliac.
 DT    9412
 AU    Miura T; Meguro T; Yamada K; Watanabe K; Honda M; Department of
       Pediatrics, Haga Red Cross Hospital, Tochigi,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):22 (abstract no. 381A). Unique
       Identifier : AIDSLINE ICA10/94371523
 AB    OBJECTIVE: HIV-1 isolates can be grouped into 6 clades (A-F), and they
       are divided into two major groups; the GPGR structure as North
       American/European type and the GPGQ structure as African/Thai type.
       Analysis of over 150 cases of Japanese hemophiliacs in NIH, Japan shows
       only clade B HIV, North American/European type but no African/Thai type.
       We report a case with the GPGQ structure in the V3 loop. CASE: A
       16-year-old Japanese hemophiliac was admitted to our hospital because of
       convulsion. HIV was isolated and he was diagnosed to have HIV
       encephalopathy. RESULTS: The amino acid sequence of the V3 loop domain
       of the isolated virus revealed the GPGQ structure, but it was different
       from two genotypes in Thailand. Analysis of DNA homology and
       phylogenetic tree revealed that the virus belonged to the North
       American/European type. The anti-PND (principal neutralization domain)
       antibodies in this patient's serum had the highest reactions to MN type.
       DISCUSSION: The isolated virus has the GPGQ structure, but its codon
       usage was similar to that of clade B virus. Interestingly, the codon
       usage of the GPGQ core sequence was identical with that of clade E
       consensus virus, but not clade B virus. The investigation of tropism of
       the virus and the follow-up will be critical for clearing up the
       particular change of the virus.
 DE    Adolescence  Amino Acid Sequence  Case Report  Hemophilia/COMPLICATIONS
       Human  HIV Antibodies/IMMUNOLOGY  HIV Envelope Protein gp120/*GENETICS
       HIV Infections/COMPLICATIONS/MICROBIOLOGY
       HIV-1/CLASSIFICATION/*GENETICS/IMMUNOLOGY/ISOLATION & PURIF  Japan  Male
       Molecular Sequence Data  Peptide Fragments/*GENETICS  Phylogeny  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).