Document 1092
 DOCN  M94A1092
 TI    Influence of different flow cytometers, monoclonal antibodies, two and
       three color labelling on the CD-4 cell count in HIV infection.
 DT    9412
 AU    Knechten H; Holmgren C; Knickmann M; Ruback C; Praxiszentrum, Aachen,
       Germany.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):210 (abstract no. PB0855). Unique
       Identifier : AIDSLINE ICA10/94371483
 AB    INTRODUCTION: Measurement of absolute CD4 cell count still is very
       unsatisfactory but of importance for therapy, diagnostic and staging in
       HIV infection. OBJECTIVE: In an ongoing study lymphocyte subsets of 200
       HIV infected individuals are measured parallely with two different flow
       cytometers, different commercial monoclonal antibodies and with a direct
       two and three color immunfluorescence labelling. The aim ist to evaluate
       the influence of these parameters on the absolute CD4 cell count.
       Furthermore practicability, advantages and desadvantages of the two
       different methods in routine work will be evaluated. METHODS: Parallel
       measurement of peripheral EDTA blood of 200 HIV infected individuals
       with a Becton Dickinson flow cytometer (FACScan), Becton Dickinson
       monoclonal antibodies as a two color reagent kit (IMK Plus reagent) and
       with an Ortho flow cytometer (CYTORON ABSOLUTE) and Ortho monoclonal
       antobodies as a three color reagent kit (ORTHO TRIO). RESULTS: The
       results will be presented. DISCUSSION AND CONCLUSIONS: Flow cytometry is
       the most common method for lymphocyte phenotyping but areliable absolute
       CD4 cell count does not yet exist. Thus, the knowledge of trends, such
       as lower or higher cell counts, dim or bright antigen expression, with
       different flow cytometers, monoclonal antibodies or two three color
       labelling are of great importance since the absolute CD4 cell count is a
       leading marker for progression in HIV disease and has diagnostic an
       therapeutic consequences. Furthermore the practicability of different
       methods in routine work is an important factor.
 DE    Antibodies, Monoclonal/*DIAGNOSTIC USE  *Flow Cytometry  *Fluorescent
       Antibody Technique  Human  HIV Infections/*IMMUNOLOGY  *Leukocyte Count
       T-Lymphocyte Subsets  *T4 Lymphocytes  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).