Document 1118
 DOCN  M94A1118
 TI    Binding of AZT to lipid membranes.
 DT    9412
 AU    Gabev EE; Gabev EB; Bulg. Ac. Sci., Sofia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):205 (abstract no. PB0832). Unique
       Identifier : AIDSLINE ICA10/94371457
 AB    OBJECTIVE: To evaluate the binding of AZT to neutral and negatively
       charged lipid membranes with or without reduced dipole potential (Vd) as
       possible explanation of targeted AZT toxicity to neuro- and blood cells
       especially when coadministered with some drugs. METHODS: Binding of AZT
       to lipid membranes was evaluated by direct surface potential (SP) and Vd
       measurements of lipid monolayers using 241 Am ionizing method and by ANS
       fluorescence in liposomes. Phloretin was used to reduce Vd. RESULTS: AZT
       up to 1mM binds weakly to lipid membranes with negative SP but
       dramatically at 0.5mM after 100mV reduction of Vd. AZT does not binds to
       neutral lipid membranes even after 40% reduction of Vd. CONCLUSIONS: 1.
       Inherent high negative SP of neuro- and blood cells are suggested as
       responsible for AZT toxicity to these cells. 2. Coadministration of AZT
       with drugs recognized to reduce Vd of cell membranes (antipyretics,
       antitumorals, etc.) may strongly increase AZT toxicity to cells with
       negative SP rather than those with less negative surface potential.
 DE    Lipid Bilayers/*METABOLISM  Liposomes  Membrane Potentials
       Zidovudine/*METABOLISM  MEETING ABSTRACT

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