Document 1136
 DOCN  M94A1136
 TI    The effect of HIV infection on measles antibody.
 DT    9412
 AU    Brunell PA; Vimal V; Courville T; Israele V; Daar E; Sandhu M; Ahmanson
       Pediatric Center, Cedars-Sinai Medical Center, Los; Angeles, California.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):200 (abstract no. PB0814). Unique
       Identifier : AIDSLINE ICA10/94371439
 AB    OBJECTIVE: To study the effect of HIV infection on immunologic memory
       and antibody avidity. METHOD: Sera from age matched HIV infected (HIV)
       and normal (Norm) infants (Inf) (n = 9 and 21), children (Child) (n = 17
       and 49) and adults (Ad) (n = 21 and 27) were tested by ELISA for measles
       antibody (MAb). Avidity index (AI) was determined with 8M urea.
       Comparisons of ratios was by Fisher's exact test and means by two sample
       t-test or analysis of variance. p < or = .01 was considered significant.
       RESULTS: Mean MAb of HIV and Norm were significantly different for Inf,
       635 +/- 349 and 1083 +/- 188; Child, 249 +/- 156 and 848 +/- 241; and Ad
       687 + 220 and 1067 +/- 305, respectively. The mean AI of HIV and Norm
       were significantly different for Inf, 18 +/- 12 and 38 +/- 16, and
       Child, 9 +/- 8 and 43 +/- 16, but not for Ad 45 +/- 17 and 52 +/- 16 (p
       = .18). The AI of Ad was significantly greater than that of Inf or
       Child. Three of 17 HIV children were MAb negative at entry into the
       study; 4 of 14 subsequently seroreverted. The decline in MAb was not
       arrested by reimmunization. Two of 3 seronegatives seroconverted, one
       transiently and 2 of 9 seropositives boosted transiently after
       reimmunization. DISCUSSION AND CONCLUSIONS: HIV Inf had a poorer MAb
       response to immunization than Norm infants indicative of impairment of
       their initial response to measles. Poor immunologic memory was
       manifested by loss of MAb and poor responses to reimmunization. The
       latter together with low AI suggests that antibody maturation was
       impaired in Inf and Child. Damage to the follicular dendritic cells
       (FDC) by HIV can best explain these findings. FDC selects B lymphocytes
       making high avidity antibody for perpetuation. Adults who were infected
       with HIV after measles appeared to be less affected.
 DE    Adult  Antibodies, Viral/*ANALYSIS  Antibody Affinity  Child
       Enzyme-Linked Immunosorbent Assay  Human  HIV Infections/*IMMUNOLOGY
       Immunization, Secondary  Immunologic Memory  Infant  Measles/PREVENTION
       & CONTROL  Measles Virus/*IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).