Document 1138
 DOCN  M94A1138
 TI    Nevirapine (nev) is less toxic to progenitor cells (BFU-E) than AZT or
       ddI.
 DT    9412
 AU    Weinberg RS; Chusid ED; Galperin Y; Gerits P; Sacks H; Mt Sinai School
       of Medicine, NY, NY 10029.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):200 (abstract no. PB0813). Unique
       Identifier : AIDSLINE ICA10/94371437
 AB    AZT and ddI inhibit HIV by inhibiting viral reverse transcriptase (RT).
       AZT causes anemia and both AZT and ddI inhibit blood BFU-E in vitro. Nev
       is a nonnucleoside inhibitor of RT now being tested in combination with
       AZT and ddI. To determine to the effect of nev on BFU-E, 0.01 to 100
       microM nev was added to methyl progenitor cell cultures from HIV+
       patients. 0.01 to 10 microM nev did not significantly inhibit the number
       or size of BFU-E. In some cultures, 50 to 100 microM nev inhibited BFU-E
       100%. 10 microM AZT and ddI inhibited BFU-E 100% and 50%. Stem cell
       factor (SCF), interleukin-3 (IL-3), and hemin (HE) were added to
       cultures with inhibitory doses of AZT, ddI, and nev. SCF and HE were
       potent stimulators of BFU-E resulting in increased numbers of colonies
       compared with antiviral drugs alone and controls. When SCF, HE, and IL-3
       were combined, growth of BFU-E was often greater than the sum of the
       colonies with each factor alone. HGFs were most effective in cultures
       with only one antiviral drug. Combination of AZT, ddI, and nev totally
       inhibited growth in most cultures and HGFs did not improve growth
       significantly. Thus, nev is less inhibitory than AZT and ddI and HGFs
       increase growth in cultures containing inhibitory doses of AZT, ddI, and
       nev. However, when AZT, ddI, and nev are combined, HGFs are less
       effective. HGFs may be less effective in patients treated with triple
       drug therapy than in those receiving single antiviral drugs.
 DE    Antiviral Agents/*TOXICITY  Cell Division/DRUG EFFECTS  Cells, Cultured
       Comparative Study  Didanosine/*TOXICITY  Erythroid Progenitor
       Cells/*DRUG EFFECTS  Human  *HIV Seropositivity  Pyridines/*TOXICITY
       Zidovudine/*TOXICITY  MEETING ABSTRACT

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