Document 1161
 DOCN  M94A1161
 TI    HIV free sperm preparation from HIV carrier's semen.
 DT    9412
 AU    Ando Y; Kakimoto K; Yoshioka A; Honda M; Wakayama Med. Col., Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(2):195 (abstract no. PB0792). Unique
       Identifier : AIDSLINE ICA10/94371414
 AB    OBJECTIVE: HIV is known for sexually transmited virus, but no effective
       method of HIV free sperm preparation from HIV carrier's semen, use for
       artificial insemination of husband (AIH). METHODS: On informed consent,
       collected HIV carrier's semens were treated 4 layer uncontinuous Percoll
       gradients, and each fruction of the gradients was tested for sperm
       count, contaminated cell count. HIV p24 antigen detection by
       co-cultivation with normal man's PBL. HIV provirus by PCR and HIV RNA by
       RT-PCR. RESULTS: From specific gravity 1110 fruction, no HIV was
       detected by all above detective methods and cotaminated cells were
       disappeared. In same fruction, sperm was prepared enough for AIH.
       DISCUSSION AND CONCLUSION: This sperm preparation method from HIV
       carrier's semen possible diminishes HIV infectivity at AIH.
 DE    Human  HIV/*ISOLATION & PURIF  HIV Core Protein p24/ANALYSIS  HIV
       Infections/*MICROBIOLOGY  Insemination, Artificial, Homologous  Male
       Semen/*MICROBIOLOGY  *Specimen Handling  Spermatozoa/*MICROBIOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).