Document 2280
 DOCN  M94A2280
 TI    Sulfated colominic acid: an antiviral agent that inhibits the
       infectivity of human immunodeficiency virus type I in vitro.
 DT    9412
 AU    Yang D; Ohta Y; Yamaguchi S; Tsukada Y; Haraguchi Y; Liu HY; Amagai H;
       Hoshino H; Department of Hygiene and Virology, Gunma University School
       of; Medicine, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):34 (abstract no. 111A). Unique
       Identifier : AIDSLINE ICA10/94370295
 AB    OBJECTIVES: Colominic acids were sulfated under different conditions and
       the antiviral activities against human immunodeficiency virus type 1
       (HIV-1) were examined. MATERIALS AND METHODS: MT-4 cells were infected
       with HIV-1 and expression of HIV-1-antigen was detected by IFA after 4
       days of cultivation. Syncytium formation assay was used to examine
       effects of compounds on early steps of HIV-1 infection. M8166 cells were
       inoculated with HIV-1 [GUN-1] (an isolate obtained from a Japanese
       hemophilia B patient with AIDS) in the presence of the compounds for 24
       or 48 hrs and the number of syncytia formed was counted using a
       microscope. HIV-1 production was measured by ELISA using p24 MoAb and
       serum of an AIDS patient and by reverse transcriptase assay. APTT of
       plasma was measured in the presence of the sulfated colominic acids by
       using an automated machine. RESULTS: Sulfated colominic acids,
       containing 6-11.8% sulfate, blocked the expression of HIV-1-antigens at
       1.0 microgram/ml in MT-4 cells that had been infected with HTLV-IIIB.
       These agents also completely blocked viral replication as assessed by
       reverse transcriptase assay. The drugs inhibited syncytium formation
       upon co-cultivation of MOLT-4 cells (clone 8) with MOLT-4/HTLV-IIIB
       cells. The compounds abolished the production of HIV-1 p24 antigen in
       culture medium of PBL. Sulfated colominic acids also inhibited infection
       of M8166 cells with HIV-1 [GUN-1]. The compounds prolonged APTT at 100
       micrograms/ml, but did not prolong at 10 micrograms/ml. Heparin and
       dextran sulfate markedly prolonged APTT at 1 microgram/ml. CONCLUSIONS:
       In these different assay systems, sulfated colominic acids displayed a
       strong anti-HIV-1 activity in vitro. They did not damage the cells at a
       concentration of 100 micrograms/ml, nor prolonged APTT at concentrations
       of up to 10 micrograms/ml, suggesting that they may have little side
       effect in vivo.
 DE    Antiviral Agents/*PHARMACOLOGY  Cell Line  Fluorescent Antibody
       Technique  Giant Cells/DRUG EFFECTS  Human  HIV Antigens/ISOLATION &
       PURIF  HIV-1/*DRUG EFFECTS  Polysaccharides/*PHARMACOLOGY  Sulfates
       Virus Replication/DRUG EFFECTS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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