Document 2328
 DOCN  M94A2328
 TI    Prevalence of antibodies to herpes simplex virus in saliva among
       Japanese female bath-house workers.
 DT    9412
 AU    Ichikawa S; Tsukano K; Okumura S; Zhang ZQ; Kihara M; Toba M; Soda K;
       Dept. of Public Health, Yokohama City Univ. School of Medicine,; Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):329 (abstract no. PC0252). Unique
       Identifier : AIDSLINE ICA10/94370247
 AB    OBJECTIVES: We established a sensitive ELISA method (ABC-ELISA) to
       detect the antibodies against herpes simplex virus (HSV) in saliva, and
       observed the prevalence among female bath-house workers. MATERIALS &
       METHODS: Paired specimens of serum and saliva were collected from 138
       healthy nurse students. Salivary specimens were collected from 191
       female bath-house workers. After centrifugation, clear saliva were
       collected and stored at 40 degrees C until testing. Serum and saliva
       were tested with ABC-ELISA method by using kits of avidin-biotin complex
       (VECTASTAIN) for detection of antibodies to HSV. RESULTS & DISCUSSION:
       All saliva specimens from bath-house workers were negative in ELISA test
       (ELAVIA MIXT) for anti-HIV. But, the prevalence of HSV antibody in
       saliva among this population was 84.3% (161/191). The positive rates of
       HSV antibody by age were 65% in the 20-29 age group, 89% in the 30-39
       age group and 94% in the > or = 40 age group. The prevalence of HSV
       antibody in saliva among the youngest bath-house worker group was
       significantly higher than that of nurse students (43.5%). Collection of
       blood specimens in epidemiological surveillance is not easy. Though it
       is necessary to improve sensitivity of ABC-ELISA method for testing of
       saliva, the mentioned results suggested that saliva was useful for
       detection of antibodies to HSV in epidemiological studies.
 DE    Adult  Antibodies, Viral/*ANALYSIS  *Enzyme-Linked Immunosorbent Assay
       Female  Herpes Simplex/*EPIDEMIOLOGY  Human  HIV Antibodies/ANALYSIS
       Japan/EPIDEMIOLOGY  *Mass Screening  Population Surveillance
       Prostitution/*STATISTICS & NUMER DATA  Saliva/*IMMUNOLOGY
       Simplexvirus/*IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).