Document 2330
 DOCN  M94A2330
 TI    HIV surveillance method by salivary HIV-antibody testing.
 DT    9412
 AU    Meesiri S; Tussana P; Jugsudee A; Eiamtrakul S; Supakalin P; Sirisopana
       N; Singharaj P; Armed Forces Research Institute of Medical Sciences
       (AFRIMS),; Bangkok, Thailand.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):329 (abstract no. PC0251). Unique
       Identifier : AIDSLINE ICA10/94370245
 AB    OBJECTIVE: To assess sensitivity and specificity of salivary
       HIV-antibody tests as a surveillance method in young men entering the
       Royal Thai Army. METHODS: Paired serum and saliva specimens were
       collected from 21-year-old men enlisted by the Royal Thai Army in
       November 1993 from Chiengmai and Bangkok metropolitan areas. Saliva
       specimen was obtained using the Omni-Sal saliva collection device. Serum
       specimen was obtained by venipuncture. Serum specimens were tested using
       EIA1 (Abbott HIV-1/HIV-2) and GPA (Serodia; Fujirebio, Japan) assays.
       Reactive serum specimens were tested by Western blot assay (HIV Blot
       2.2, Diagnostic Biotechnology, Singapore). Saliva specimens were tested
       by EIA1, GPA, and EIA2 (Detect-HIV HIV-1/HIV-2) assays. RESULTS: From a
       total of 1,295 men it was found that 107 men had seropositive for
       HIV-antibody. There were 9 false-positive cases by GPA in saliva and 1
       false-negative case by EIA1 in saliva. Serum tests did not show any
       false result and were used as reference standard methods. Comparatively,
       saliva test methods yielded the following percentages of
       sensitivity/specificity: GPA 100/99.24; EIA1 99.07/100; and EIA2
       100/100, respectively. Similarly, the percentages for predictive value
       of positive/predictive value of negative are: GPA 92.24/100; EIA1
       100/99.91; and EIA2 100/100, respectively. CONCLUSIONS: Saliva specimen
       collection is easy to perform, non-invasive, safe to use, and thus,
       provides alternative method to blood collection. Preliminary test
       results demonstrated excellent sensitivity and specificity in the
       detection of salivary HIV-antibody by EIA assays. Consequently, it is
       suggested that at least two assays by identical or different salivary
       HIV-antibody tests be performed in HIV surveillance. Further study is
       still needed before the approaches could finally be implemented as
       alternative methods to serum assays.
 DE    Adult  AIDS Serodiagnosis/*METHODS  Human  HIV Antibodies/*ANALYSIS  HIV
       Seropositivity/DIAGNOSIS/*EPIDEMIOLOGY  HIV-1/IMMUNOLOGY
       HIV-2/IMMUNOLOGY  Male  Military Personnel/*STATISTICS & NUMER DATA
       *Population Surveillance  Predictive Value of Tests  Saliva/*IMMUNOLOGY
       Thailand/EPIDEMIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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