Document 2417
 DOCN  M94A2417
 TI    HIV-1 genotypes in infants and their mothers, Bangkok, Thailand.
 DT    9412
 AU    Samakoses R; Phramonkutklao Hospital, Bangkok, Thailand.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):309 (abstract no. PC0164). Unique
       Identifier : AIDSLINE ICA10/94370158
 AB    OBJECTIVE: To study HIV-1 genotypes in infants born at Phramongutklao
       (PMK) hospital, Bangkok, Thailand and their mothers. METHODS: Between
       Dec 92 and Jan 94, PCR was performed on blood specimens from 21 infants
       born to HIV(+) mothers at PMK hospital. Blood samples from 10 mothers
       were also tested. PCR was performed using HIV-1 specific primers from
       gag and env regions. Universal primers were used to detect HIV-1 DNA.
       Seven genotype specific primers (3 gag and 4 env) were used to
       provisionally differentiate env genotype B from env genotype E in
       infected Thais. RESULTS: During the study period there were 64
       deliveries to HIV(+) mothers. Twenty-one (33%) infants (age range 20 to
       201 days, median = 60) were tested by PCR. PCR was positive in 5 (25%);
       all were HIV-1 genotype E. The 5 PCR(+) infants were between 1 and 2 mos
       of age at the time of detection. PCR was (+) in 9 (90%) of 10 mothers
       tested. Seven HIV strains in mothers were genotype E and 2 were
       unclassified. All of the PCR(+) infants were symptomatic by 2 months of
       age (CDC classification P2A). CD4 counts were available from all 16
       PCR(-) infants and were within the normal range. CD4 counts in 2 of 5
       PCR(+) infants were below the age-adjusted 5th percentile. CONCLUSIONS:
       HIV-1 env genotype E was apparently the predominant strain in vertically
       transmitted HIV-1 infection in Bangkok during the study period. Infants
       infected with genotype E in this study became symptomatic at an early
       age.
 DE    Female  Genes, env  Genes, gag  Genotype  Human  HIV
       Seropositivity/*MICROBIOLOGY  HIV-1/*GENETICS/ISOLATION & PURIF  Infant,
       Newborn  Polymerase Chain Reaction  Pregnancy  Pregnancy Complications,
       Infectious/*MICROBIOLOGY  RNA, Viral/ANALYSIS  Thailand  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).