Document 2546
 DOCN  M94A2546
 TI    HIV infection alters cytokine kinetics in stimulated PBMC.
 DT    9412
 AU    Fahey JL; Fan J; Breen E; Derzic S; Nguyen T; Martinez-Maza O; Dept. of
       Microbiology and Immunology, UCLA School of Medicine; 90024-1747.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):28 (abstract no. 085A). Unique
       Identifier : AIDSLINE ICA10/94370029
 AB    OBJECTIVE: To characterize HIV-associated cytokine dysregulation
       contributing to activation and deficiency in the immune system, the gene
       expression and production of 4 cytokines were investigated. METHODS:
       Cytokine expression and secretion by peripheral blood mononuclear cells
       (PBMC) from HIV+ (mean CD4/mm3 = 390) and HIV- persons was determined.
       mRNA was extracted from cell pellets and culture supernatant (SN)
       collected at 0, 4, 24 and 72 hours post-stimulation with PHA or anti-CD3
       antibody. Relative amounts of cytokine mRNA were determined by RT-PCR
       and cytokine SN concentrations determined by ELISA. RESULTS: Cytokine
       mRNA expression (TNF alpha, IL-4, IL-2 and IFN gamma) in HIV+
       PHA-stimulated PBMC consistently peaked at 4 hours, in contrast to HIV-
       PBMC which peaked at 24 hours. SN peaked at 72 hours. Mean peak IFN mRNA
       was higher in HIV+ but IL-4 was lower. Anti-CD3 stimulated HIV+ PBMC
       also tended to show peak cytokine mRNA expression at 4 hours.
       CONCLUSION: PBMC of HIV+ persons respond to stimulation with more rapid
       kinetics of cytokine expression. Differences in individual cytokines
       were observed. Such altered kinetics may result in inappropriate
       cytokine production, contributing to the disruption of normal immune
       function seen in HIV disease.
 DE    Cytokines/GENETICS/*PHARMACOKINETICS  Enzyme-Linked Immunosorbent Assay
       Human  HIV Infections/*IMMUNOLOGY  Interferon Type II/PHARMACOKINETICS
       Interleukin-2/PHARMACOKINETICS  Interleukin-4/PHARMACOKINETICS
       Leukocytes, Mononuclear/METABOLISM  Lymphocyte Transformation
       Polymerase Chain Reaction  RNA, Messenger/ISOLATION & PURIF  Tumor
       Necrosis Factor/PHARMACOKINETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).