Document 2610
 DOCN  M94A2610
 TI    Synergistic effect of zidovudine (AZT) and sho-saiko-to (SST) a
       traditional kampo drug on HIV replication in lymphocyte cultures.
 DT    9412
 AU    Maitra U; Mohan VP; Klein EB; Inada Y; Lange M; St. Luke's-Roosevelt
       Hosp. Ctr., Columbia University, Div. of; Virology, New York, NY 10019.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):265 (abstract no. PB0491). Unique
       Identifier : AIDSLINE ICA10/94369965
 AB    OBJECTIVE: SST is a traditional Kampo (oriental) herbal medicine
       demonstrated to have immuno-modulatory effects as well as to inhibit HIV
       reverse transcriptase. We tested the effects of AZT and SST singly and
       in different combinations on PHA stimulated lymphocyte cultures (LC)
       infected in vitro with either HIV or plasma from viremic patients.
       METHODS: HIV infected LC were exposed to AZT at 0.5, 1.0 and 3.0 uM AZT
       concentration, and to SST at 25, 50 and 100 ug/ml and the combinations
       of the two. Supernatants from the cultures were assayed for p24 antigen
       on the 7th day and from plasma cultures after 21 days. RESULTS: No
       significant reduction of p24 antigen was seen at 1.0uM AZT or 100ug/ml
       SST alone whereas an 80 fold reduction occurred at 3.0 uM AZT. Synergy
       on combining AZT and SST was observed as follows: AZT at conc'n of 0.5
       uM, 1.0 uM and 3.0 uM together with 25 ug/ml of SST reduced p24 antigen
       levels to 83, 244 and 732 fold respectively, compared to untreated
       cultures. In plasma culture synergy occurred only at AZT conc'n of 1.0
       uM or greater on day 21 with a 70 fold reduction in p24 antigen.
       CONCLUSIONS: AZT and SST at concentrations pharmacologically achievable
       which by themselves are not inhibitory, act synergistically in
       inhibiting HIV either in lymphocytes infected with HIV or in plasma
       cultures from viremic patients.
 DE    Cells, Cultured  Dose-Response Relationship, Drug  Drug Synergism
       Drugs, Chinese Herbal/*PHARMACOLOGY  Human  HIV/*DRUG EFFECTS  HIV Core
       Protein p24/ANALYSIS  Lymphocytes/MICROBIOLOGY  Virus Replication/*DRUG
       EFFECTS  Zidovudine/*PHARMACOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).