Document 2623
 DOCN  M94A2623
 TI    Early diagnostic markers of HIV infection in infants born to HIV
       seropositive mothers.
 DT    9412
 AU    Wasi C; Foongladda S; Sutthent R; Likanonsakul S; Louisirirotchanakul S;
       Chearskul S; Thongcharoen P; Department of Microbiology, Faculty of
       Medicine Siriraj Hospital,; Mahidol University, Bangkok, Thailand.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):262 (abstract no. PB0476). Unique
       Identifier : AIDSLINE ICA10/94369952
 AB    OBJECTIVE: To establish the diagnostic tests for diagnosis of HIV
       infection in infants born to HIV seropositive mothers. METHODS: The 108
       infants born to HIV seropositive mothers were followed up in the Special
       Pediatric Clinic, Siriraj Hospital during April 1992 to December 1993. A
       total of 275 subsequent blood samples were investigated for anti-HIV-1
       by the two ELISA (Genelavia & Wellcozyme), PA (Fujirebio); p24 antigen
       by ELISA after acid treatment for immune complex dissociation (Coulter)
       and nested PCR for gag region (SK 380/SK390, SK38/SK39). RESULTS: The 65
       infants showed seroreversion at the age of 6 to 15 months and assumed to
       be non-infected cases. The 30 infants shown symptoms compatible to
       Pediatric AIDS. The 13 infants were asymptomatic but positive for PCR,
       and/or p24 Ag and anti-HIV. PCR were positive in 95% (43/45 samples) of
       AIDS case and 93% (29/31) in asymptomatic, while p24 Ag was positive in
       70% of AIDS case and 58% of asymptomatic ones. DISCUSSION AND
       CONCLUSION: PCR is the most sensitive test for early diagnosis of HIV
       infection in infants. The positive PCR was nearly 100% at the age of
       newborn to 6 months. The p24 Ag after acid treatment is a practical test
       but gave only 58% positive in asymptomatic cases.
 DE    *AIDS Serodiagnosis  Enzyme-Linked Immunosorbent Assay  Female
       Follow-Up Studies  Human  HIV Antibodies/BLOOD  HIV Core Protein
       p24/BLOOD  HIV Infections/DIAGNOSIS/IMMUNOLOGY/*TRANSMISSION  HIV
       Seropositivity/DIAGNOSIS/IMMUNOLOGY/*TRANSMISSION  HIV-1/*IMMUNOLOGY
       Infant  Infant, Newborn  Male  Polymerase Chain Reaction  Pregnancy
       Pregnancy Complications, Infectious/*DIAGNOSIS/IMMUNOLOGY  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).