Document 2673
 DOCN  M94A2673
 TI    New fast low-cost method of HIV diagnostics based on carbon-conjugated
       antigens.
 DT    9412
 AU    Shevalier A; Mikhailov M; Nikolaeva I; Turchinsky M; Institute of
       Bioorganic Chemistry, Moscow, Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):250 (abstract no. PB0430). Unique
       Identifier : AIDSLINE ICA10/94369902
 AB    OBJECTIVE: To develop the new cost-effective appropriate technology of
       HIV antibody detection. METHODS: Fragments of HIV genome have been
       obtained by PCR from HIV-1 genome. HIV-recombinant proteins (containing
       parts of gag and env) were expressed in E. coli pMal vectors, purified
       and attached to the carbon particles. Recombinant proteins were absorbed
       onto nitrocellulose membrane, undiluted sera were spotted and after
       washing carbon-HIV proteins conjugates were added. HIV antibody were
       indicated by black spots. RESULTS: Method was evaluated with
       representative collection of HIV positive (130 specimens) and HIV
       negative (600 specimens) sera. The sensitivity was 100%; the specificity
       was 99,5%. Total assay time is approximately 10 min. DISCUSSION AND
       CONCLUSIONS: Method provides simple easy to read, low-cost,
       high-sensitive and specific HIV antibody detection. This technique can
       be used effectively for non-instrumental diagnostics in developing
       countries.
 DE    AIDS Serodiagnosis/*ECONOMICS  Carbon  Collodion  Cost-Benefit Analysis
       Human  HIV Antibodies/*BLOOD  HIV Antigens/DIAGNOSTIC USE/*IMMUNOLOGY
       HIV Seropositivity/*DIAGNOSIS/ECONOMICS/IMMUNOLOGY  Membranes,
       Artificial  Predictive Value of Tests  Reagent Kits,
       Diagnostic/ECONOMICS  Recombinant Proteins/DIAGNOSTIC USE/IMMUNOLOGY
       MEETING ABSTRACT

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