Document 2683
 DOCN  M94A2683
 TI    A newly-developed immunofluorescence assay for simultaneous detection of
       antibodies to human immunodeficiency virus type 1 and type 2.
 DT    9412
 AU    Tanabe-Tochikura A; Singh MT; Tsuchie H; Zhang J; Mattunda SM; Paladin
       FJ; Kurimura T; Dept. of Pathology, Osaka Univ., Suita, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):249 (abstract no. PB0424). Unique
       Identifier : AIDSLINE ICA10/94369892
 AB    OBJECTIVE: Immunofluorescence assay (IFA) which is capable of
       simultaneously detecting antibodies against closely related HIV-1 and
       HIV-2 was established and evaluated for the ability to serologically
       discriminate HIV-1 and HIV-2 infections. METHODS: IFA employing three
       types of cells, HIV-1-infected (U937/LAI), HIV-2-infected
       (Multinucleated giant cells which were formed by cocultivation of
       MOLT-4#8 and MOLT-4/GH-1) and uninfected (HeLa) cells whose morphology
       varies within one field of microscopy was performed. Serum specimens
       from 95 HIV-1-infected, 26 HIV-2-infected and 3 HIV-1/HIV-2
       dually-infected individuals and 106 seronegative blood donors were
       tested using above mentioned cells. RESULTS: All the samples from
       individuals infected with HIV were confirmed to contain antibodies to
       HIV-1 and/or HIV-2. None of the sera from blood donors were positive.
       Serum specimens from HIV-1-infected or HIV-2-infected individuals were
       diagnosed as infections with HIV-1 (85/95) and HIV-2 (22/26),
       respectively. Other 14 (10/95 and 4/26) were shown to be seropositive
       for both HIV-1-infected and HIV-2-infected cells. DISCUSSION AND
       CONCLUSION: Our results of newly-developed IFA was capable of
       distinguishing most (88%) of HIV-1 and HIV-2 infections at one time.
       This assay appears to be useful for screening as well as for confirming
       of both HIV-1 and HIV-2 infections simultaneously.
 DE    AIDS Serodiagnosis/*METHODS  Cell Line  *Fluorescent Antibody Technique
       Giant Cells/MICROBIOLOGY  Hela Cells  Human  HIV Antibodies/*BLOOD  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  Predictive Value of Tests  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).