Document 2684
 DOCN  M94A2684
 TI    Purification, processing, and diagnostic use of the HIV-1 Gag gene
       products expressed in Escherichia coli.
 DT    9412
 AU    Shinagawa H; Saitoh A; Morimoto M; Nakata A; Research Institute for
       Microbial Diseases, Osaka University,; Suita, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):248 (abstract no. PB0423). Unique
       Identifier : AIDSLINE ICA10/94369891
 AB    OBJECTIVE: To purify and evaluate the various Gag proteins of HIV-1
       produced in Escherichia coli as antigens for the diagnosis and
       prognosis. METHODS: HIV-1 Gag proteins, p17, p24, p15, and p55, were
       overproduced under the control of T7 promoter in E. coli and purified to
       near homogeneity. Two-fold increasing amounts from 5 ng to 320 ng of
       each of the Gag proteins were dotted on to the PVDF membrane and the
       antigen dots were immunologically reacted with 1:100 dilution of the
       sera of 40 HIV-1 carriers (AC 35, ARC 1, and AIDS 4). RESULTS: p55
       reacted with all the 40 sera of HIV carriers. p17, p24, and p15 reacted
       with 37, 35, and 34 of the 40 sera of HIV carriers, respectively. In
       general, the sera of ARC and AIDS reacted more weakly with p17, p24, and
       p55 than those of AC. The reaction of p55 was especially strong and that
       of p15 was the weakest. In fact, p55 showed the strongest reaction in
       all cases. DISCUSSION AND CONCLUSIONS: p55 is the most useful antigen
       among the four Gag proteins for detection of Gag antibody in sera. The
       methods to produce the HIV-1 Gag precursor and to process it to mature
       products in vivo and in vitro were established.
 DE    Acquired Immunodeficiency Syndrome/DIAGNOSIS/IMMUNOLOGY  AIDS
       Serodiagnosis/*METHODS  AIDS-Related Complex/DIAGNOSIS/IMMUNOLOGY
       Escherichia coli/GENETICS  Gene Products, gag/DIAGNOSTIC USE/*GENETICS
       Human  HIV Antigens/DIAGNOSTIC USE/*GENETICS  HIV Core Protein
       p24/DIAGNOSTIC USE/*GENETICS  HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/*GENETICS/IMMUNOLOGY  Predictive Value of Tests  Prognosis
       Promoter Regions (Genetics)  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).