Document 2690
 DOCN  M94A2690
 TI    Simple and sensitive detection of antibodies to HIV-1 using
       chemiluminescent immunochromatography.
 DT    9412
 AU    Hasegawa A; Andoh A; Kawabata K; Ashihara Y; FUJIREBIO INC., Research
       Laboratories, Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):247 (abstract no. PB0417). Unique
       Identifier : AIDSLINE ICA10/94369885
 AB    OBJECTIVE: The establishment of a simple and sensitive method for the
       multiple detection of antibodies to HIV-1 and HBs that would eliminate
       tedious procedures and elaborate instrumentation. METHOD: This method is
       based upon chemiluminescent immunochromatography using glassfiber strip
       and instant film for photo detection. The test strip is shown in Fig. 1.
       After dropping 50 microliters of sample on the sample zone, the
       immunoreaction is started by the developing solution, and the following
       enzyme reaction is carried out with CSPD as chemiluminescent substrate.
       The strip is developed and the enzyme is reacted for 30 minutes. The
       strip is then exposed on the instant film for 30 seconds. RESULTS: Low
       titer sample with 2(5) dilution positive by particle agglutination (PA)
       test showed positive result on the film. In this method, 15 positive
       samples in the PA test showed positive result, but 5 negative samples in
       the PA test did not react. DISCUSSION AND CONCLUSION: In the present
       method, we were able to achieve a simple and sensitive assay with
       results easily read by naked eye. Moreover, with this method the
       simultaneous detection of antibodies to both HIV-1 and HBs for primary
       blood screening is now possible. Assay conditions still need to be
       improved to obtain more rapid assay with shortened reaction time.
       TABULAR DATA, SEE ABSTRACT VOLUME.
 DE    AIDS Serodiagnosis/*METHODS  *Chemiluminescence  Chromatography/*METHODS
       Human  HIV Antibodies/*BLOOD  HIV Envelope Protein gp41/IMMUNOLOGY  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Predictive
       Value of Tests  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).