Document 2691
 DOCN  M94A2691
 TI    Evaluation of peptide-based HIV ELISA kits.
 DT    9412
 AU    Nishimura H; Watanabe T; Hirakawa H; Kageyama S; Kurimura T; Nippon
       Shoji Kaisha, Ltd. Osaka, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):247 (abstract no. PB0418). Unique
       Identifier : AIDSLINE ICA10/94369884
 AB    OBJECTIVE: The aim of this study is to verify whether or not NB-02(for
       detection of HIV-1 and/or-2 antibodies) and NB-06 (for differentiation
       between HIV-1 and -2 antibodies) kits are useful as screening and
       differentiation tests using an exclusive automatic analyzer. METHODS:
       Two kinds of kit(mentioned above) and the exclusive automatic analyzer
       were obtained from BIOCHEM IMMUNOSYSTEMS INC. (Canada). The principle of
       these kits is ELISA for detection of IgG-Ab. Experimental items:
       1)Evaluation of NB-02 for its specificity, reproducibility and
       sensitivity; 2) Etiological study on HIV-1 and/or HIV-2 infections in
       sera of several races in combination with NB-02 and NB-06. RESULTS: (1)
       Evaluation of NB-02; Specificity: Anti HIV-positive samples tested (n =
       75) were seropositive by this kit, and the seropositivity was markedly
       correlated with that by the Serodia-HIV kits (PA method) which are
       widely used as a screening test in Japan; Reproducibility: C.V. of the 3
       lots were 3.4, 6.0, 3.2%, respectively; Sensitivity: HIV-positive
       samples (n = 75) prediluted 10(4)-fold exhibited to be highly reactive
       with the percentage of 96%. (2) It was found that Zambian (n = 28),
       Thai(n = 11) and Japanese(n = 19) sera tested were all infected with
       HIV-1 alone, whereas Senegalese sera tested (n = 11) were mostly
       infected with HIV-2, except for one sample (co-infection with HIV-1 and
       -2). DISCUSSION AND CONCLUSIONS: As to the performance of NB-02 and
       NB-06 kits and the exclusive automatic analyzer: 1) The operation with
       autosystem was simple; 2) The determination was rapid and accurate; 3)
       The sensitivity and the reproducibility were satisfactory. From these
       results NB-02 and NB-06 using the exclusive automatic analyzer are
       useful for routine assay in hospitals and clinical laboratories.
 DE    AIDS Serodiagnosis/*METHODS  Comparative Study  Enzyme-Linked
       Immunosorbent Assay/*METHODS  Human  HIV Antibodies/*BLOOD  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  Peptide Fragments/*IMMUNOLOGY  Predictive Value of
       Tests  *Reagent Kits, Diagnostic  Reproducibility of Results  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).