Document 2698
 DOCN  M94A2698
 TI    Comparative study of results obtained by ELISA in detecting anti-HIV
       antibodies in human serum using various kits.
 DT    9412
 AU    Negut E; Mazilu E; Butur D; Balteanu M; Ciufecu C; Vrinceanu L;
       Cantacuzino Institute, Bucharest, Romania.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):245 (abstract no. PB0411). Unique
       Identifier : AIDSLINE ICA10/94369877
 AB    OBJECTIVE: Study of operational performances of various types of ELISA
       kits obtained on sera from infantile population in Romania in the
       National Reference Centre, Cantacuzino Institute. METHODS: The study was
       carried out on 1000 sera: each group of 500 sera was tested on two ELISA
       kits, the indirect type (VIRONOSTIKA-ORGANON, HIV1 env Peptide
       EIA-LABSYSTEMS and respectively RAPID ELAVIA MIXT--PASTEUR and
       RECVIH--CUBA) and on one competitive ELISA kit (WELLCOZYME HIV1 Rec type
       VK 56/57 WELLCOZYME). The confirmatory investigation was carried out by
       western-blot (Diagnostic Biotechnology Du-Pont) using WHO criteria.
       RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: Sera from
       Romanian subjects were correctly detected by ELISA kits under
       consideration, similar O.C. with those reported by WHO laboratories
       being obtained. In case of out-of-date kits validation is not a
       sufficiently reliable criterium for their use (too many obtained results
       are found in the grey zone and an exceedingly high dispersion of data is
       reported when delta values are calculated).
 DE    *AIDS Serodiagnosis  Comparative Study  *Enzyme-Linked Immunosorbent
       Assay  Human  HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY  Infant
       Predictive Value of Tests  Quality Control  *Reagent Kits, Diagnostic
       Romania  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).