Document 2702
 DOCN  M94A2702
 TI    Approaches to the creation of antibody to HIV-1 reference panel for
       quantitative estimation of enzyme immunoassay diagnostic kits.
 DT    9412
 AU    Kanev AN; Gutova EA; Vorobyova MS; Lomanova GA; Shalaev FY; Institute of
       Molecular Biology, Moscow, Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):244 (abstract no. PB0407). Unique
       Identifier : AIDSLINE ICA10/94369873
 AB    To increase the reability of EIA where positive and negative sera to HIV
       antibodies are used to discriminate different diagnostic kits we offer
       to form the panel based on sera with low concentration of specific to
       HIV-1 antibodies so that in the range of optical density (OD) index
       directly adjoining to cut-off the quantitive presence of sera would be
       maximum. The panel consists of 40 samplers: 10 members and reactive for
       anti HIV-1 and 24 human sera that contain no detectable anti HIV. The
       panel is derived from several human plasma sources from the European and
       Asian parts of Russia. We have worked out preparation method of stable
       sera with low concentration of HIV antibodies from infected human sera
       to form the panel in accordance with the histogram of theoretical
       distribution of their OD index on EIA results. In accordance with the
       physico-chemical analysis data the composition of stabilizing solution
       and freeze-drying conditions are selected in order to prepare stable
       potent form of sera. The sera stability was checked in thermodegradation
       test at 4 degrees C, 20 degrees C, 37 degrees C and 45 degrees C during
       nine months. The samples in the panel retain its potency for at least 24
       months provided they are stored at 4-8 degrees C. Basing on the
       distribution of sera OD index histogram we of diagnostic kits on the
       ground of a statistical treatment of EIA data.
 DE    AIDS Serodiagnosis/*METHODS  Comparative Study  Human  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  *Immunoenzyme
       Techniques  Predictive Value of Tests  *Reagent Kits, Diagnostic
       Reference Standards  MEETING ABSTRACT

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