Document 2706
 DOCN  M94A2706
 TI    Field comparison of line-immunoassays with western blot.
 DT    9412
 AU    Maskill W; Tamashiro H; Belsey E; M'Boup S; Wasi C; Liomba G;
       Gershy-Damet G; Fairfield University Melbourne.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):243 (abstract no. PB0401). Unique
       Identifier : AIDSLINE ICA10/94369869
 AB    OBJECTIVE: To determine under field conditions the accuracy of
       line-immunoassays (LIA's) for confirming seropositivity for HIV-1 and
       HIV-2 as compared to the Western Blot (WB). METHODS: Sera from four
       countries which were according to WHO WB interpretation criteria
       positive for HIV-1 (n = 650), HIV-2 (n = 35), both HIV-1 and HIV-2 (n =
       63), negative (n = 571) or indeterminate (n = 47) were tested by two
       LIA's (Inno-Lia and Pepti-Lav). Results of the LIA's were interpreted
       according to the criteria provided by the test manufacturers. RESULTS:
       The Inno-Lia and Pepti-Lav tests showed a 98.3% and 98.2% agreement
       respectively with the WB for HIV-1 positive sera. Both LIA's showed 100%
       agreement with the WB for HIV-2 positive sera. Both assays showed
       approximately only a 60% agreement with the WB for sera positive for
       both HIV-1 and HIV-2 with the remaining sera being positive only for
       HIV-1. The LIA's found the majority of WB indeterminate sera to be
       negative, however both tests showed poor specificity due to WB negative
       sera showing indeterminate reactions. DISCUSSION AND CONCLUSION:
       Although the Inno-Lia and Pepti-Lav tests show a high level of agreement
       of WB positive sera, find the majority of WB indeterminate samples
       non-reactive, and produce a lower number of dually reactive sera, both
       assays produce a significant number of indeterminate reactions with WB
       negative sera. The occurrence of these indeterminate reactions may limit
       the ability of these tests to completely replace the WB.
 DE    AIDS Serodiagnosis/*METHODS  *Blotting, Western  Comparative Study
       Human  HIV Antibodies/BLOOD  HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY  *Immunoassay  Predictive Value of
       Tests  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).