Document 2708
 DOCN  M94A2708
 TI    Reduction of false positive reaction by a combination assay of two
       different test principles (EIA and PA) for HIV antibody screening.
 DT    9412
 AU    Imai M; Hayashi T; Kondo M; Saito T; Suzuki K; Sugimoto K; Kihara M;
       Nojiri N; Juji T; Nishioka K; Kanagawa Prefectural Institute of Health,
       Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):242 (abstract no. PB0399). Unique
       Identifier : AIDSLINE ICA10/94369867
 AB    OBJECTIVES: Majority of HIV antibody screening positive samples showed
       false positive reaction and cofirmatory negative or indeterminate
       results by western blot (WB). To reduce these false positive reactions
       by single screening test, a combination assay of two different test
       principles EIA and particle agglutination assay (PA) were employed for
       HIV antibody screening. METHODS: The first HIV antibody test was
       performed by EIA and a second HIV antibody test was performed by PA if
       the first screening was positive. Both screening test (EIA and PA)
       positive samples were tested by WB and some samples were also tested for
       DNA by PCR. RESULTS AND CONCLUSIONS: Among 50,000 serum samples, 61 were
       positive by EIA. Of these 27 were positive by PA, and of the 27 both
       screening positive, 24 were confirmed HIV antibody positive by WB.
       Therefore, our combination assay of two different test principles is
       practical for HIV antibody screening becouse it avoids many false
       positive results if only one screening test is used before the WB test.
 DE    Agglutination Tests/*METHODS  AIDS Serodiagnosis/*METHODS  Blood Banks
       Blotting, Western  Comparative Study  False Positive Reactions  Human
       HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY  *Immunoenzyme Techniques
       Japan  Polymerase Chain Reaction  MEETING ABSTRACT

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       protected by Copyright Law (Title 17, U.S.Code).