Document 2713
 DOCN  M94A2713
 TI    Differentiation of HIV-1 infection cross reacting with HIV-2 from real
       HIV-1 and HIV-2 double infection by serological absorption test.
 DT    9412
 AU    Hayashi T; Kondo M; Saito T; Hayami M; Nishioka K; Imai M; Kanagawa
       Prefectural Institute Of Health, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):241 (abstract no. PB0394). Unique
       Identifier : AIDSLINE ICA10/94369862
 AB    OBJECTIVES: Some samples of sera from HIV-1 infected cases showed
       serological cross reaction with HIV-2 causing confusion of
       serodiagnosis. Therefore we tried to differentiate these cases from real
       double infection with both viruses. METHODS: Sera were tested by
       agglutination of HIV-1 and HIV-2 gelatin particle (PA), western blot,
       pepti LAV for HIV-1 and HIV-2 and cross absorption with HIV-1 and HIV-2.
       HIV progenomes were tested by specific primers of HIV-1 and HIV-2.
       RESULTS: We selected two HIV infected sera in Japan strongly positive by
       PA, western blot and pepti LAV for both HIV-1 and HIV-2. HIV-1 absorbed
       off both HIV-1 and HIV-2 reactivity but HIV-2 could absorb HIV-2
       reactivity but not HIV-1. Double infected West-African serum with HIV-1
       and HIV-2 showed only corresponding reactivity which was absorbed off
       with corresponding antigen. These results were also confirmed by type
       specific PCR test. CONCLUSIONS: HIV-1 infection cross reacting with
       HIV-2 was differentiated from real HIV-1 and HIV-2 double infection by
       our serological cross absorption test, which is proven to be highly
       specific and useful for serodiagnosis.
 DE    Absorption  *AIDS Serodiagnosis  Blotting, Western  Comparative Study
       Cross Reactions/*IMMUNOLOGY  Diagnosis, Differential  Enzyme-Linked
       Immunosorbent Assay  Human  HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY  Polymerase Chain Reaction
       Predictive Value of Tests  MEETING ABSTRACT

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