Document 2714
 DOCN  M94A2714
 TI    Detection of combination-type particle agglutination assay for detection
       of HIV-1 and HIV-2 antibodies.
 DT    9412
 AU    Nishimura Y; Nakamura K; Kido K; Matsui T; Miyakoshi H; Fujino R;
       FUJIREBIO INC., Research Laboratories, Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):241 (abstract no. PB0393). Unique
       Identifier : AIDSLINE ICA10/94369861
 AB    OBJECTIVE: We have developed a combination-type gelatin particle
       agglutination assay (HIV 1/2combi.) for the detection of HIV-1 and HIV-2
       antibodies using recombinant antigens. Antibodies to HIV-1 and/or HIV-2
       were detected at 1:32 final dilution of samples. METHOD: Three different
       antigens were mixed and co-coated on gelatin particles. Sensitized
       particles were evaluated for their potential in a screening kit. To
       determine the sensitivity and specificity of HIV 1/2 combi., 178 HIV-1
       and/or HIV-2 positive specimens, and 7600 blood donors samples were
       tested. RESULTS AND CONCLUSION: The sensitivity of HIV 1/2combi. was
       100% for HIV-1 and HIV-2 positive specimens, and was higher than other
       kits using BBI's HIV-1 seroconversion panels. The specificity of our kit
       was 99.85% for the blood donor samples. Further studies are still needed
       to improve the specificity of HIV 1/2combi. Using HIV 1/2combi., the
       detection of HIV-1 and HIV-2 antibodies is accurate, rapid and easily
       performed.
 DE    *Agglutination Tests  *AIDS Serodiagnosis  Blood Donors  Human  HIV
       Antibodies/*BLOOD  HIV Antigens/IMMUNOLOGY  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  Predictive Value of Tests  Recombinant
       Proteins/IMMUNOLOGY  MEETING ABSTRACT

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