Document 2724
 DOCN  M94A2724
 TI    Sample Addition Monitor gives improved safety of blood supply with
       Wellcozyme HIV 1+2 assay.
 DT    9412
 AU    Povey J; Beckford U; Rayment F; Evans CJ; Murex Diagnostics Ltd.,
       Dartford, Kent, U.K.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):239 (abstract no. PB0386). Unique
       Identifier : AIDSLINE ICA10/94369851
 AB    OBJECTIVE: Errors in addition of sample, manually or with automatic
       dispensers, affect the sensitivity of assays. A sample diluent was
       therefore developed, which would confirm that sample had been added to
       the well, thereby confirming that the protocol was followed. RESULTS:
       The diluent is green/brown and changes to blue when serum or plasma is
       added to it. This change is easily seen and can be recorded
       spectrophotometrically (absorbance increases from 0.05 to 0.35). Assay
       sensitivity has been improved with the following bleeds detected of BBI
       HIV-1 seroconversion panels; BBI R3, L2, Q5, I2, S2 and of NABI panels;
       151-2C, 171-2 C and 121-2C. The sensitivity of HIV-2 detection has also
       been increased. CONCLUSION: The inclusion of a Sample Addition Monitor
       facilitates the confirmation that sample has been added to the well.
       Mistakes can be easily identified and rectified thereby improving the
       safety of blood supply.
 DE    AIDS Serodiagnosis/*INSTRUMENTATION  Blood Banks  *Blood Donors  Human
       HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  Immunoenzyme Techniques/*INSTRUMENTATION  Predictive
       Value of Tests  Quality Control  Specimen Handling/INSTRUMENTATION
       Spectrophotometry/INSTRUMENTATION  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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