Document 2725
 DOCN  M94A2725
 TI    Performance of the Red-Dot HIV 1 & 2 assay.
 DT    9412
 AU    Abdel-Hamid M; Constantine NT; Dept. Pathology, University of Maryland
       School of Medicine,; Baltimore.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):239 (abstract no. PB0387). Unique
       Identifier : AIDSLINE ICA10/94369850
 AB    OBJECTIVES: To determine the suitability and performance of a new rapid
       assay to detect antibodies to HIV 1 & 2 in a hospital setting in
       Baltimore, MD. METHODS: A total of 91 sera (50 EIA negative, 39 EIA
       repeatedly reactive/Western blot (WB) confirmed, and 2 WB indeterminate)
       were tested by the Red-Dot HIV 1 & 2 rapid assay (CDI, CA, USA). In
       addition, 9 samples were included that were EIA false positive (WB
       negative). The Red-Dot test is a rapid membrane based assay which
       incorporates a synthetic peptide antigen and a protein A colloidal gold
       conjugate. Samples which exhibited discrepant results between the two
       screening tests were retested by all assays to ensure that technical
       error had not occurred. RESULTS: 39 sera produced reactive results by
       both tests and were confirmed by Western blot (WB). The Red-Dot test
       produced 3 false positive results (detected 56 of 59 true negatives),
       and no false negative results (0/39), thus yielding a specificity of 95%
       and a sensitivity of 100%. Both of the WB indeterminate samples produced
       reactive results by EIA, while only one produced a reactive result by
       the Red-Dot. DISCUSSION AND CONCLUSIONS: The Red-Dot HIV 1 & 2 rapid
       assay was easy to perform, required just minutes to complete, exhibited
       an excellent sensitivity and an acceptable specificity. This study
       should be expanded to include a larger sample size in order to verify
       the assay's performance.
 DE    *AIDS Serodiagnosis  *Blotting, Western  Human  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  *Immunoenzyme Techniques  Predictive Value of Tests
       MEETING ABSTRACT

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