Document 2730
 DOCN  M94A2730
 TI    Clinical evaluation of an automated assay (Vidas, Biomerieux) for
       determination of p24 antigenemia.
 DT    9412
 AU    Barin F; Buzelay L; Vernet G; Goudeau A; Laboratoire de Virologie, CHRU
       Bretonneau, Tours, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):238 (abstract no. PB0381). Unique
       Identifier : AIDSLINE ICA10/94369845
 AB    OBJECTIVE: To evaluate the sensitivity and specificity of a new
       automated assay for determination of HIV1 p24 antigenemia. METHODS: The
       VIDAS assay was evaluated by comparison to 2 licensed p24 assays
       (HIVAG-1, Abbott, and Vironostika HIV1-Ag, Organon). The sensitivity was
       studied on 1) a panel of 28 samples from the Societe Nationale de
       Transfusion Sanguine (SNTS), 2) serial dilutions of the WHO standard
       HIV1 preparation, and 3) 170 consecutive samples from HIV1 seropositive
       individuals adressed to our clinical virology laboratory. Specificity is
       being tested on samples from HIV1 seronegative blood donors. RESULTS AND
       DISCUSSION: The limits of HIV1 Ag detection were 10-15 pg/ml, 20-25
       pg/ml and 35-40 pg/ml for Vironostika HIV1 Ag, Vidas, and HIVAG-1,
       respectively, when using the WHO HIV1 preparation. The Vidas and
       Vironostika assays gave similar data on the SNTS panel, whereas 2 weakly
       positive samples (20-25 pg/ml) detected by these 2 assays were negative
       by HIVAG-1. First data on the 170 clinical samples indicate that the
       sensitivities of Vidas, Vironostika, and HIVAG-1 were 89.6%, 85.1%, and
       74.6%, respectively. Specificity studies will be available for
       presentation.
 DE    AIDS Serodiagnosis/*INSTRUMENTATION  Blood Banks  *Blood Donors
       Comparative Study  France  Human  HIV Core Protein p24/*IMMUNOLOGY  HIV
       Seronegativity/IMMUNOLOGY  HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Predictive Value of Tests  Reproducibility of Results
       MEETING ABSTRACT

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