Document 2735
 DOCN  M94A2735
 TI    Cell-associated and extracellular HIV-1 antigens: variations in
       serologic enzyme-immuno assays (EIAs).
 DT    9412
 AU    Troshev O; Argirova R; Central AIDS Lab., Sofia, Bulgaria.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):237 (abstract no. PB0377). Unique
       Identifier : AIDSLINE ICA10/94369840
 AB    OBJECTIVES: To investigate the influence of HIV-1 antigen source and
       preparation upon signal-to-background ratio in three different EIAs; and
       determine how Western blot banding pattern variations reflect virus
       glycoprotein processing pathways. METHODS: Both group of antigens for
       the EIAs (ELISA, Dot-ELISA and confirmatory Western blot) were prepared
       from HIV-1 producing cell line HUT/ARV4, using various approaches and
       biochemical procedures. RESULTS: We have achieved the optimal
       signal-to-background ratio in these EIAs as follows: 1) ELISA with
       extracellular HIV-1 antigen purified in a sucrose density gradient; 2)
       Dot-ELISA with extracellular antigen precipitated with PEG-6000; 3)
       Western blot (WB) with cell-associated HIV-1 lysate-homogenate. In last
       case the envelope precursor polyprotein gp160 has regularly appeared
       along with its cleavage products (gp120 and gp41), whereas a WB with
       extracellular virus has always reflected gp120 and gp41 only.
       CONCLUSIONS: The extracellular HIV antigen is more appropriate in a
       screening serology (ELISA or Dot-ELISA) because of significantly reduced
       non-specific background. The cell-associated HIV-1 antigen in Western
       blot enables to detect precisely all the three envelope-specific
       antibodies, directed to the precursor, external and transmembrane
       glycoproteins of HIV-1.
 DE    Blotting, Western  Comparative Study  Enzyme-Linked Immunosorbent
       Assay/METHODS  Gene Products, env/BLOOD  Human  HIV Antigens/*BLOOD  HIV
       Envelope Protein gp120/BLOOD  HIV Envelope Protein gp41/BLOOD  HIV
       Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  *Immunoenzyme
       Techniques  Predictive Value of Tests  Protein Precursors/BLOOD  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).