Document 2737
 DOCN  M94A2737
 TI    Comparative study of p24 antigen capture assays in human
       immunodeficiency virus-1 (HIV) seropositives.
 DT    9412
 AU    Gotoh M; Matsuda J; Gohchi K; Tsukamoto M; Saitoh N; Department of
       Medicine, Teikyo University School of Medicine,; Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):236 (abstract no. PB0373). Unique
       Identifier : AIDSLINE ICA10/94369838
 AB    OBJECTIVE: The detection of p24 antigen has been widely used clinically
       employing a commercially available test kit-conventional test (Abott,
       USA), but the positivity of p24 in HIV-positive patients has been found
       to be relatively low by this method possibly due to the immune complex
       formation (p24-anti p24). We compared the sensitivity of the
       conventional and new test kit--ICD-PREP Kit (Coulter Corporation, USA)
       which enable the immune complex dissociate in conjunction with anti-p24
       antibody (ENVCORE, Abott). SUBJECTS: 65 patients with hemophilia,
       including 35 infected with HIV and 20 healthy volunteers were studied.
       RESULTS: p24 was positive in 6/35 (17%) by conventional test and 16/35
       (45%) by ICD-PREP. Four of seven patients (positive for p24 by
       conventional test) who were negative for anti-p24 antibody were negative
       for p24 by ICD-PREP kit. In contrast, p24 was revealed to be positive
       even in patients positive for anti-p24 antibody by the new method. 57%
       patients were positive for p24 in overall by two methods. All p24
       positive patients were positive for anti-gp41 antibody. CONCLUSION: From
       these, we should examine p24 employing both conventional and ICD-PREP
       tests to detect p24 antigen precisely.
 DE    Antigen-Antibody Complex/IMMUNOLOGY  AIDS Serodiagnosis/*METHODS
       Comparative Study  Hemophilia/IMMUNOLOGY  Human  HIV Core Protein
       p24/*BLOOD  HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       Predictive Value of Tests  MEETING ABSTRACT

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