Document 2738
 DOCN  M94A2738
 TI    Clinical evaluation of the Abbott HIV AG-1 monoclonal EIA with and
       without immune complex disruption (ICD) on a panel of asymptomatic HIV-1
       seropositive patients.
 DT    9412
 AU    Cunningham PH; Carr AD; Nichols D; Penny R; Cooper DA; Centre for
       Immunology, St. Vincent's Hospital, Sydney, Australia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):236 (abstract no. PB0372). Unique
       Identifier : AIDSLINE ICA10/94369837
 AB    AIMS: To assess the performance of the HIV1 AG monoclonal antigen assay
       with and without Immune Complex Disruption (ICD) for the detection of
       HIV core antigen in serial serum samples collected from asymptomatic
       HIV-1 infected individuals enrolled in a placebo (n = 106) vs
       anti-retroviral (n = 103) controlled clinical trial. METHODS: A mean of
       5 serial serum (n = 1076) samples collected from a total of 209 patients
       were tested with monclonal HIV1 AG (Abbott, IL, USA) with and without
       ICD. Data was compared to historical polyclonal HIV1 AG (Abbott, IL,
       USA) results and detection limits of the two monoclonal procedures were
       assessed. RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. 123/209 (58.9%)
       patients had no specimen positive by any method. 19/209 (9.1%) of
       patients had the first serial serum specimen tested positive by ICD
       alone. 53/209 (25.3%) of patients had one or more specimens positive by
       ICD and negative by the other methods. 20/53 (37.7%) of these patients
       showed trends in p24 titre over time with ICD. CONCLUSION: The Abbott
       HIV1 AG monoclonal ICD procedure facilitates the disruption of the
       antibody-antigen complex allowing the p24 to become available for
       detection by EIA. It is appears to permit earlier detection of HIV-1
       antigen in individuals with progressive disease. Monoclonal detection of
       HIV-1 antigen appears to be a sensitive and specific and easy to use
       method suitable for routine measurement of circulating viral load.
 DE    Antigen-Antibody Complex/*IMMUNOLOGY  Comparative Study  Human  HIV Core
       Protein p24/*BLOOD  HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  *Immunoenzyme Techniques  Predictive Value of Tests
       MEETING ABSTRACT

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