Document 2739
 DOCN  M94A2739
 TI    p24 in serum from HIV-1 seropositives.
 DT    9412
 AU    Hashida S; Hashinaka K; Nishikata I; Oka S; Shimada K; Saitoh A;
       Shinagawa H; Takamizawa A; Ishikawa E; Department of Biochemistry,
       Medical College of Miyazaki, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):236 (abstract no. PB0374). Unique
       Identifier : AIDSLINE ICA10/94369836
 AB    OBJECTIVE: To test the positivity of p24 assayed by a new enzyme
       immunoassay (EIA) in sera from HIV-1 seropositives. METHOD: An
       ultrasensitive two-site enzyme immunoassay (two-site complex transfer
       enzyme immunoassay) was developed. The immune complex consisting of
       2,4-dinitrophenyl (DNP)-biotinyl-bovine serum albumin-anti-p24 Fab', p24
       and anti-p24 Fab'-beta-D-galactosidase (GAL) conjugate was trapped onto
       anti-DNP IgG-coated polystyrene balls (PS), eluted with DNP-L-lysine and
       transferred to streptavidin-coated PS. GAL activity bound to the last PS
       was assayed by fluorometry. RESULTS: The detection limit of p24 was 0.24
       ng/l using 10 microliters of serum. p24 was detected in 50 out of 79
       sera from seropositives (25 out of 50 sera from asymptomatic carriers
       (AC) and 25 out of 29 sera from advanced patients) and all negative in
       100 sera from seronegatives. Thus, the sensitivity and specificity of
       the assay were 64% and 100%, respectively. Furthermore, after
       acid-treatment of serum, the sensitivity increased to 75% (68% even for
       AC) with no change in the specificity. DISCUSSION AND CONCLUSIONS: The
       sensitivity of the new assay was apparently superior to those of
       commercially available kits. The high positivity of p24 assayed by the
       method in sera may allow us to use p24 a useful surrogate marker.
 DE    Human  HIV Core Protein p24/*BLOOD  HIV
       Seropositivity/CLASSIFICATION/*DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY
       *Immunoenzyme Techniques  Predictive Value of Tests  MEETING ABSTRACT

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