Document 2747
 DOCN  M94A2747
 TI    HIV 1 specific LTR- and gag-sequences with essential defects in
       seronegative injecting drug users.
 DT    9412
 AU    Dennin RH; Chen Z; Meissner C; Institut fur Medizinische Mikrobiologie,
       Medizinische; Universitat, Lubeck, FRG.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):234 (abstract no. PB0367). Unique
       Identifier : AIDSLINE ICA10/94369828
 AB    OBJECTIVE: There are numerous publications dealing controversely with
       the phenomenon of anti-HIV 1 seronegative individuals although positive
       for HIV 1 specific sequences. We especially aimed to look for HIV 1
       specific sequences of the LTR- and gag-region. METHODS: Liver tissues
       and small samples of heart punctured blood from eleven injecting drug
       users (IDU) at autopsy were available. DNA was extracted from the liver
       tissue; serum was used for anti-HIV 1 & 2 assays. For nested PCR we used
       proved primers specific for sequences of the LTR- & gag-region and
       different combinations of both primer sets bridging the 3'LTR-5'LTR
       junction site of the circle structure. RESULTS: Four out of 11 DNA
       extracts repeatedly yielded positive signals when PCR with primers of
       the gag-region was performed. However, certain primers of the U5' region
       failed, although control assays with DNA from acutely HIV 1-infected H9
       lymphocytes showed the expected fragments with a sensitivity of 1 to 10
       copies/assay. DISCUSSION AND CONCLUSIONS: In 4/11 anti-HIV 1
       seronegative individuals at high risk HIV 1 sequences were found with
       obvious functional defects. Within the LTR region (an) essential
       mutation(s) or even a deletion could be located that is confined to the
       U5' stretch.
 DE    Diagnosis, Differential  Genes, gag/*GENETICS  Human  *HIV
       Seronegativity  HIV-1/*GENETICS  Liver/PATHOLOGY  Polymerase Chain
       Reaction/METHODS  Predictive Value of Tests  Repetitive Sequences,
       Nucleic Acid/*GENETICS  Substance Abuse, Intravenous/*BLOOD  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).