Document 2751
 DOCN  M94A2751
 TI    NASBA HIV-1 RNA amplification compared to HIV-1 RNA-PCR on plasma or
       serum samples: a Belgian field evaluation.
 DT    9412
 AU    Vandamme AM; Van Dooren S; Kok W; Goubau P; Fransen K; Kievits T;
       Desmyter J; Rega Institute, Leuven-Belgium.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):233 (abstract no. PB0363). Unique
       Identifier : AIDSLINE ICA10/94369824
 AB    The presence of HIV-1 RNA in the plasma or serum of European and African
       patients was monitored using RNA-PCR and the new HIV-1 NASBA system
       involving an isothermal amplification. Identical RNA extraction
       procedures, provided by the NASBA system, were used for both methods.
       Both NASBA and RNA-PCR are more sensitive than p24 for the detection of
       HIV-1 free virus in blood: 18 of the 35 p24 tested seropositives were
       p24 negative while only 2 were negative for both NASBA and RNA-PCR
       (Table). The detection limit for HIV-1 RNA was slightly better for
       NASBA: in 8 microliters plasma or serum, 0.01 CCID50 of spiked HIV-IIIB
       was reported positive for NASBA but indeterminate (ID) for RNA-PCR. Two
       seronegative samples were NASBA and RNA-PCR positive or indeterminate.
       This was probably due to a sample carryover contamination, which can be
       avoided by more careful lab practises. There was no cross reactivity
       with HIV-2 or HTLV-I. The extraction method used allowed us to detect
       HIV-1 RNA equally well in plasma on heparine or on EDTA. TABULAR DATA,
       SEE ABSTRACT VOLUME.
 DE    Belgium  Comparative Study  Gene Amplification/*GENETICS  Human  HIV
       Core Protein p24/BLOOD  HIV Infections/*DIAGNOSIS/MICROBIOLOGY  HIV
       Seronegativity  HIV Seropositivity/DIAGNOSIS/MICROBIOLOGY
       HIV-1/*GENETICS  Polymerase Chain Reaction/*METHODS  Predictive Value of
       Tests  RNA, Viral/*BLOOD/GENETICS  MEETING ABSTRACT

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