Document 2753
 DOCN  M94A2753
 TI    Detection of HIV-1 proviral DNA by PCR using AMPLICOR HIV-1 test kit.
 DT    9412
 AU    Suzuki H; Yoshihara N; Fukushima K; Honda M; Yamazakik S; Fukutake;
       Meguro T; Yamada K; Oka S; AIDS Research Center, NIH, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):232 (abstract no. PB0357). Unique
       Identifier : AIDSLINE ICA10/94369822
 AB    OBJECTIVE: AMPLICOR HIV-1 kits, which had been developed as an HIV-1
       provirus detection test by PCR method, have been evaluated for its
       clinical diagnostic application. We have compared AMPLICOR HIV-1 with
       virus isolation and HIV-1 antibody test. METHODS: (1) HIV-1 antibody
       test (Particle agglutination and Western Blot), (2) Virus isolation, (3)
       PCR by AMPLICOR HIV-1 test kit. SAMPLES: Two hundred sixty-two of HIV-1
       antibody positive and 67 of HIV-1 negative blood samples derived from
       hemophiliacs, who had received blood products. Three of new born infants
       from HIV-1 sero-positive mothers. RESULTS: The results from PCR were
       consistent with those from antibody test and clinical aspects.
       Thirty-nine of HIV-1 antibody positive samples have been tested by PCR
       and virus isolation. Thirty-nine of 39 (100%) were positive by PCR and
       12 of 39 (30.8%) were positive by virus isolation. One of three new born
       infants from HIV-1 positive mothers were known HIV-1 infection by PCR.
       We did not see the silent infection in hemophiliacs. CONCLUSIONS:
       AMPLICOR HIV-1 is considered as useful clinical diagnostic for HIV-1
       proviral DNA detection and it suggested that the kit would be useful for
       diagnosis of infants from sero-positive mothers.
 DE    Adult  AIDS Serodiagnosis  Comparative Study  DNA, Viral/*BLOOD  Female
       Hemophilia/COMPLICATIONS  Human  HIV Antibodies/BLOOD  HIV
       Infections/CONGENITAL/COMPLICATIONS/*DIAGNOSIS/MICROBIOLOGY
       HIV-1/GENETICS/*ISOLATION & PURIF  Infant, Newborn  *Polymerase Chain
       Reaction  Pregnancy  Pregnancy Complications, Infectious
       Proviruses/GENETICS/*ISOLATION & PURIF  *Reagent Kits, Diagnostic
       Sensitivity and Specificity  Viremia/*MICROBIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).