Document 2754
 DOCN  M94A2754
 TI    Detection of HIV RNA and DNA in saliva by RT-PCR and PCR under different
       conditions.
 DT    9412
 AU    Ando S; Kakizawa J; Yamamoto Y; Saito K; Oka S; Muller WE; Ushijima H;
       AIDS Research Center, NIH, Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):232 (abstract no. PB0356). Unique
       Identifier : AIDSLINE ICA10/94369821
 AB    OBJECTIVE: To detect HIV genes in saliva on inactivated [disinfected]
       filter paper disks--after safe transport--by using PCR technique.
       MATERIALS AND METHODS: We have further developed and improved this
       method for use with saliva samples. Eighty-three saliva from patients
       with AC, ARC and AIDS [42 hemophiliacs; 10 homosexuals; 10
       heterosexuals] were collected and applied to filter disks, dried and
       disinfected by elevated temperatures. HIV DNA, HIV RNA, occult blood and
       HIV antibody in saliva were analyzed by PCR, RT-PCR, strip method and
       particle agglutination, respectively. RESULTS: Occult blood, HIV Ab, HIV
       RNA and HIV DNA were positively detected from the saliva. The results
       did not depend on the stage of the disease or the route of infection.
       DISCUSSION: The filter paper disk method can be used not only for the
       detection of Abs but also for HIV-genome from saliva samples.
 DE    DNA, Viral/*ANALYSIS  Evaluation Studies  Human  HIV/GENETICS/*ISOLATION
       & PURIF  HIV Antibodies/ANALYSIS  Occult Blood  Polymerase Chain
       Reaction/INSTRUMENTATION/*METHODS  RNA, Viral/*ANALYSIS
       Saliva/CHEMISTRY/IMMUNOLOGY/*MICROBIOLOGY  Salivary Proteins/ANALYSIS
       Specimen Handling/METHODS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).