Document 2755
 DOCN  M94A2755
 TI    Belgian multicentre quality control on the performance of selected
       primer sets for diagnostic HIV-1 PCR. Belgian AIDS Reference
       Laboratories.
 DT    9412
 AU    Vandamme AM; Fransen K; Burtonboy G; Debaisieux L; Marissens D; Rega
       Institute, Leuven, Belgium.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):232 (abstract no. PB0359). Unique
       Identifier : AIDSLINE ICA10/94369820
 AB    To evaluate the performance of their diagnostic HIV-1 DNA PCR, 8 Belgian
       AIDS Reference Laboratories established a multicentre quality control.
       The centres tested a set of Belgian and African patient samples,
       collected in Belgium, and the British Medical Research Council (MRC)
       HIV-1 PCR reference reagent kit, containing plasmid HIV-1 DNA at several
       dilutions in human carrier DNA with appropriate negative controls. No
       false positive results were reported. All centres proved to be able to
       detect 1 to 2 copies of HIV-1 DNA. Among the 17 Belgian and African
       HIV-1 seropositives, some centres reported up to 4 indeterminate
       results, mainly due to failure of the SK38-39, SK68-69 (Ou et al. 1988)
       and/or gag881-882 (Simmonds et al. 1990) primers and to a bad performing
       algorithm. Only the H1POL4235-4538 nested pol primer set, developed by
       one of the centres, correctly identified the tested HIV-1 positive and
       negative samples. To improve their performance, the centres decided to
       evaluate these pol primers as a reference primer set and to standardize
       the testing algorithm. On 10 additional Belgian and African patient
       samples, all centres achieved a sensitivity and specificity of 100% when
       adapting a standardized algorithm based on 3 HIV-1 primer sets, one of
       which is the H1POL4235-4538 primer set.
 DE    Africa  Algorithms  Belgium  Comparative Study  DNA Primers/*STANDARDS
       DNA, Viral/*ANALYSIS/GENETICS  False Positive Reactions  Genes, gag
       Genes, pol  Human  HIV Infections/MICROBIOLOGY
       HIV-1/GENETICS/*ISOLATION & PURIF  Polymerase Chain Reaction/*STANDARDS
       Quality Control  Reagent Kits, Diagnostic  Reference Standards
       Sensitivity and Specificity  MEETING ABSTRACT  MULTICENTER STUDY

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