Document 2756
 DOCN  M94A2756
 TI    Value of urinary HIV antibody in staging in patients with AIDS.
 DT    9412
 AU    Vardinon N; Yust I; Yavorkovsky E; Zeldis I; Stanescu R; Burke M; Tel
       Aviv Medical Center, Israel.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):231 (abstract no. PB0355). Unique
       Identifier : AIDSLINE ICA10/94369819
 AB    OBJECTIVE: In order to assess the diagnostic value of urine testing in
       HIV seropositive (SP) individuals, we examined urine and serum specimens
       in parallel to peripheral blood lymphocyte (PBL) populations from 74 HIV
       SP subjects. METHODS: Urine specimens were tested by EIA Abbott 3rd
       generation (3-G-EIA) and modified EIA (Mod-EIA). 156 seronegative
       subjects served as controls. Positive results were confirmed by Western
       blot (WB). For Mod-EIA, mouse antihuman IgG (Sigma) was substituted as
       the conjugate in the Abbott kit. PBL were monitored in a FACScan (BD)
       using monoclonal antibodies (Coulter) for T-cells CD3, CD4 and CD8.
       RESULTS: Sixty-nine out of 74 urine samples of SP individuals reacted in
       3-G-EIA, whereas all 74 reacted in Mod-EIA. All five cases reacting as
       false negative urine samples in 3-G-EIA had CD4 counts higher than
       900/mm2), CD4/CD8 ratio > 1 and indeterminate WB patterns with bands to
       gp160 or p24. The urine OD cut-off ratio in HIV SP subjects with CD4
       counts above 700 was significantly lower than that of all other groups
       (p < 0.005). Sensitivity and specificity of Mod-EIA were 100% and 93.6%
       respectively. DISCUSSION AND CONCLUSION: Urinary antibody using Mod-EIA
       with 3-G-EIA is a simple highly sensitive and specific test for
       screening HIV infection and assessing early stages of HIV
       seroconversion.
 DE    Acquired Immunodeficiency Syndrome/*DIAGNOSIS  Comparative Study
       Evaluation Studies  Human  HIV Antibodies/BLOOD/*URINE  HIV
       Infections/DIAGNOSIS/*URINE  HIV Seropositivity/URINE  Immunoenzyme
       Techniques  Leukocyte Count  Lymphocyte Subsets  Sensitivity and
       Specificity  MEETING ABSTRACT

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