Document 2757
 DOCN  M94A2757
 TI    Urine test for anti-HIV antibodies based on synthetic peptides.
 DT    9412
 AU    Sidorovitch IG; Ignatieva GA; Institute for immunology, Moscow, Russia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):231 (abstract no. PB0353). Unique
       Identifier : AIDSLINE ICA10/94369818
 AB    We elaborated non-invasive non-infectious test-system suitable for
       detection of anti-HIV antibodies in urine. We used at first synthetic
       ELISA test-system Peptoscreen-2 (Institute for immunology, Moscow). But
       if it is used according instruction for preparation it can not
       differentiate intact urine from HIV-infected and non-infected persons.
       To reach satisfactory results we invented two modifications. The 1-st is
       method of very simple concentration of urine immunoglobulins by
       self-salts-precipitation at freezing/thawing of intact urine. The 2-nd
       is an introduction of new peptide antigen on solid phase. After
       freezing/thawing a pellet is dissolved in 1/100 volume of PBS to initial
       volume of fresh morning urine. The sensitivity of reaction is raised
       approximately two-fold after absorption on solid phase the peptide aa.
       603-624 (gp41) instead of peptide aa. 601-616. In urine we find anti-HIV
       antibodies of IgG and IgA, but not IgM class. In some patients
       concentration of anti-virus antibodies in such preparation from urine
       was higher than in serum.
 DE    Comparative Study  *Enzyme-Linked Immunosorbent Assay  Evaluation
       Studies  Freeze Drying  Human  HIV/*IMMUNOLOGY  HIV Antibodies/*URINE
       HIV Envelope Protein gp41/*IMMUNOLOGY  HIV Infections/*URINE
       IgA/IMMUNOLOGY/*URINE  Peptide Fragments/CHEMICAL SYNTHESIS/*IMMUNOLOGY
       Precipitation  Sensitivity and Specificity  Specimen Handling/METHODS
       MEETING ABSTRACT

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