Document 2758
 DOCN  M94A2758
 TI    Saliva can show seroconversion.
 DT    9412
 AU    Sangare KA; Koffi AR; Ehouman A; Pasteur Institut, Cote d'Ivoire.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):231 (abstract no. PB0352). Unique
       Identifier : AIDSLINE ICA10/94369817
 AB    OBJECTIVE: To evaluate the ability of saliva to detect seroconversion.
       MATERIEL AND METHODS: From a population at risk for HIV infection, 250
       consenting adults were determined to be negative by the following
       assays: Abbott Recombinant HIV-1/HIV-2, 3rd generation; Enzygnost anti
       HIV-1/HIV-2; Vironostika Mixt. All subjects were free to leave the study
       at any time. Once a week saliva was collected from each subject with the
       Omni-SAL collector (Saliva Diagnostic Systems, Inc.). The specimen was
       tested with Abbott Test-Pack and Wellcozyme GACELISA on the day of
       collection. In addition a dried blood spot was collected and assayed
       according to the procedure in the Wellcozyme GACELISA protocol. If a
       positive was obtained with any test, a blood sample was taken the same
       day or as soon as possible for a Western Blot analysis. RESULTS: One
       individual had confirmed positive saliva and dried blood spot specimens
       in the third week of the study. Western Blots, run on serum samples
       obtained the next day and in the following weeks for up to two months,
       showed a progressive increase in the intensity and number of bands,
       starting with only p24 and gp160 and by two months showing, in addition
       p17, p31, gp41, p51, p55, p66 and gp120. No individuals had confirmed
       positive dried blood spot specimens that were not positive by saliva.
       CONCLUSION: Our study shows that saliva is as effective as a dried blood
       spot specimen in determining HIV positivity in early seroconversion.
 DE    Adult  Blotting, Western  Cohort Studies  Comparative Study
       Enzyme-Linked Immunosorbent Assay  Evaluation Studies  Follow-Up Studies
       Human  HIV Antibodies/*ANALYSIS/BLOOD  HIV Seropositivity/*DIAGNOSIS
       HIV-1/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY  Risk Factors  Saliva/*IMMUNOLOGY
       Salivary Proteins/*ANALYSIS  DUPLICATE PUBLICATION  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).