Document 2761
 DOCN  M94A2761
 TI    Saliva-based HIV testing in Thailand.
 DT    9412
 AU    Frerichs RR; Silarug N; Eskes N; Pagcharoenpol P; Rodklai A;
       Thangsupachai S; Wongba C; UCLA, Department of Epidemiology, University
       of California at Los; Angeles 90024-1772.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):230 (abstract no. PB0349). Unique
       Identifier : AIDSLINE ICA10/94369814
 AB    OBJECTIVE: To determine if saliva could serve as a non-invasive
       alternative to serum for testing of HIV antibodies in the on-going
       sentinel surveillance program in Thailand. METHODS: Serum and saliva
       specimens were gathered anonymously from 1,955 persons in four of 73
       sentinel sites of the national surveillance program in Thailand. Saliva
       was gathered with the Omni-Sal collection device (SDS) and analyzed with
       the GACELISA (Murex). Serum served as the gold standard and was analyzed
       with an ELISA test for screening and the WB for confirmation. Linkage
       between serum and saliva was blind to the laboratory. Also analyzed
       blind was a set of HIV+ and HIV- quality assurance samples for both
       serum and saliva. RESULTS: The sensitivity of GACELISA with saliva was
       98.0% in the field (298 HIV+), 100% after correction of errors (300
       HIV+), and 100% among the quality assurance samples (95 HIV+). The
       specificity of GACELISA was 99.4% in the field (1,653 HIV-), 99.6% after
       correction of errors (1,654 HIV-), and 100% among the quality assurance
       samples (96 HIV-). CONCLUSION: Our findings in the largest study of its
       kind ever done, demonstrate that saliva is comparable to serum for
       assessment of HIV antibodies in a surveillance program. The next step is
       to develop a simple, inexpensive device for anonymous home screening.
 DE    Comparative Study  Enzyme-Linked Immunosorbent Assay  Evaluation Studies
       Human  HIV Antibodies/*ANALYSIS/BLOOD  HIV Infections/*EPIDEMIOLOGY
       Mass Screening/*METHODS  *Population Surveillance  Saliva/*IMMUNOLOGY
       Salivary Proteins/*ANALYSIS  Sensitivity and Specificity  Single-Blind
       Method  Thailand/EPIDEMIOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).