Document 2771
 DOCN  M94A2771
 TI    Evaluation of the HIV antibody detection in oral fluid from high risk
       subjects in Thailand.
 DT    9412
 AU    Isarangura NA; Auwanit VV; Lokett R; Songsomboon S; Bhumisawasdi J;
       Department of Medical Sciences, Ministry of Public Health,; Bangkok,
       Thailand.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):229 (abstract no. PB0344). Unique
       Identifier : AIDSLINE ICA10/94369804
 AB    OBJECTIVES: To determine the feasibility of using oral fluid for the
       detection of HIV antibodies. METHOD: Matched oral fluid and serum
       samples were obtained from a hospital for drug user in Thailand. The
       Orasure device (Epitope Inc.) was used to collected oral specimens. Sets
       of oral fluid and serum were tested with the Vironostika HIV-1
       Microelisa (Organon Teknika). Repeated reactive samples and those giving
       discordant results were further confirmed by serum Western blotting
       (Epitope Inc.) RESULTS: The sensitivity and specificity of oral sample
       testing, using the results of serum testing as a gold standard, are 100%
       (84/84) and 99.5% (219/220). for HIV antibody detection. Six serum
       samples produced inconclusive results by western blot. Four of matched
       oral fluid specimens were positive and 2 were negative by EIA.
       DISCUSSION AND CONCLUSIONS: HIV antibody testing in oral fluid obtained
       by Orasure device is highly sensitive and specific. The use of an oral
       sample provide a safer and more convenient procedure than blood sample
       for HIV antibody detection with the application in surveillance as well
       as those problems on blood collection. However, retesting of blood
       sample is necessary for diagnosis purpose. Detection of low level HIV
       antibody in oral fluid need some modification of EIA system to get a
       good sensitivity and specificity. Disadvantage of this technique is a
       high cost of collecting device for oral fluid.
 DE    Blotting, Western  Comparative Study  *Enzyme-Linked Immunosorbent Assay
       Evaluation Studies  Exudates and Transudates/*IMMUNOLOGY
       Gingiva/*IMMUNOLOGY  Human  HIV Antibodies/*ANALYSIS/BLOOD  HIV
       Infections/COMPLICATIONS/*DIAGNOSIS  HIV-1/*IMMUNOLOGY  Risk Factors
       Sensitivity and Specificity  Specimen Handling/INSTRUMENTATION
       Substance Abuse, Intravenous/COMPLICATIONS  Thailand/EPIDEMIOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).