Document 2776
 DOCN  M94A2776
 TI    What is the real advantage of saliva collectors in anti-HIV testing?
 DT    9412
 AU    Ubolyam S; Phanuphak P; Fang C; Department of Microbiology Chulalongkorn
       Hospital, Bangkok,; Thailand.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):227 (abstract no. PB0339). Unique
       Identifier : AIDSLINE ICA10/94369799
 AB    OBJECTIVE: To determine the advantage of the saliva collection device,
       Omni-SAL in preserving saliva for anti-HIV testing as compared to saliva
       collected by direct spitting. METHODS: Saliva specimens from 20 each of
       HIV-infected and normal individuals were obtained by the saliva
       collection device, Omni-SAL and by direct spitting into a plastic
       container (Untreated saliva). Half of the specimen was kept at 4 degrees
       C and the other half at 37 degrees C for 0, 1, 3, 7, 14 and 21 days. At
       the end of each incubation period, aliquots were removed and stored at
       -20 degrees C until simultaneously assayed for anti-HIV activity.
       RESULTS: All Omni-SAL specimens from infected individuals tested
       positive on Abbott HIV-1/2 EIA after storage either at 4 degrees C or 37
       degrees C for up to 21 days. For untreated saliva, the anti-HIV activity
       remained fully stable for 14 days at 4 degrees C but only for 7 days at
       37 degrees C, 50% and 100% of the anti-HIV activity in the untreated
       saliva was lost after 14 and 21 days of storage respectively. All saliva
       specimens from 20 seronegative individuals were anti-HIV negative
       irrespectively of the methods of collection or the duration and
       condition of storage. DISCUSSION AND CONCLUSIONS: Saliva collection
       device, Omni-SAL has the advantage over saliva collected by direct
       spitting for anti-HIV testing in that it can preserve the anti-HIV
       activity for up to 21 days at 37 degrees C storage whereas saliva
       obtained by direct spitting is reliable for only 7 days at the same
       condition.
 DE    Comparative Study  Cryopreservation  Evaluation Studies  Human  HIV
       Antibodies/*ANALYSIS  HIV Infections/*DIAGNOSIS  Predictive Value of
       Tests  Preservation, Biological  Saliva/*IMMUNOLOGY  Salivary
       Proteins/*ANALYSIS  Specimen Handling/*INSTRUMENTATION  Temperature
       Time Factors  MEETING ABSTRACT

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