Document 2835
 DOCN  M94A2835
 TI    Effect of cryopreservation on HIV+VE lymphocytes stored for adoptive
       immunotherapy.
 DT    9412
 AU    Trickett A; Dwyer J; Tesfamariam N; St. George Hospital, Kogarah, NSW,
       Australia.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):214 (abstract no. PB0286). Unique
       Identifier : AIDSLINE ICA10/94369740
 AB    OBJECTIVE: To study the the effect of cryopreservation, storage and
       thawing on lymphocytes obtained from asymptomatic HIV+ve patients. It is
       planned that the autologous cryopreserved cells will be expanded and
       reinfused when the patient progresses towards AIDS, with the aim of
       delaying disease progression. METHODS: Blood mononuclear cells were
       obtained by leukapheresis and cryopreserved in DMSO. Samples of fresh
       and cryopreserved (> 4 months) cells were analysed for lymphocyte subset
       proportion by 3 colour immunofluorescence. Results were compared using
       paired t test. RESULTS: Expressed as % +ve in lymphocyte cluster. Mean
       +/- 1SD. n = 6. TABULAR DATA, SEE ABSTRACT VOLUME. DISCUSSION: A
       decrease in proportion of CD4+ lymphocytes was seen following
       cryopreservation. This decrease was not evident in the CD4+CD45RA+ or
       CD4+CD28- subsets. Further studies are required to determine whether HIV
       infected CD4+ cells have a survival disadvantage during the
       cryopreservation process.
 DE    *Blood Preservation  Blood Transfusion, Autologous  Cell Survival
       Comparative Study  *Cryopreservation  Human  HIV Infections/*BLOOD
       Immunophenotyping  *Immunotherapy, Adoptive  Leukapheresis  *Lymphocyte
       Subsets/IMMUNOLOGY  T4 Lymphocytes  MEETING ABSTRACT

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