Document 0140
 DOCN  M9550140
 TI    Secondary structure in solution of two anti-HIV-1 hammerhead ribozymes
       as investigated by two-dimensional 1H 500 MHz NMR spectroscopy in water.
 DT    9505
 AU    Sarma RH; Sarma MH; Rein R; Shibata M; Setlik RS; Ornstein RL; Kazim AL;
       Cairo A; Tomasi TB; Institute of Biomolecular Stereodynamics, State
       University of New; York at Albany 12222.
 SO    FEBS Lett. 1995 Jan 9;357(3):317-23. Unique Identifier : AIDSLINE
       MED/95137114
 AB    Two hammerhead chimeric RNA/DNA ribozymes (HRz) were synthesized in pure
       form. Both were 30 nucleotides long, and the sequences were such that
       they could be targeted to cleave the HIV-1 gag RNA. Named HRz-W and
       HRz-M, the former had its invariable core region conserved, the latter
       had a uridine in the invariable region replaced by a guanine. Their
       secodary structures were determined by 2D NOESY 1H 500 MHz NMR
       spectroscopy in 90% water and 10% D2(0), following the imino protons.
       The data show that both HRz-M and HRz-W form identical secondary
       structures with stem regions consisting of continuous stacks of AT and
       GT pairs. An energy minimized computer model of this stem region is
       provided. The results suggest that the loss of catalytic activity that
       is known to result when an invariant core residue is replaced is not
       related to the secondary structure of the ribozymes in the absence of
       substrate.
 DE    Base Sequence  HIV-1/*DRUG EFFECTS  Molecular Sequence Data  Nuclear
       Magnetic Resonance/*METHODS  *Protein Structure, Secondary  RNA,
       Catalytic/*CHEMISTRY/PHARMACOLOGY  Solutions  Support, Non-U.S. Gov't
       Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).