Document 0258
 DOCN  M9550258
 TI    Modification of the fungi-fluor and the genetic systems fluorescent
       antibody methods for detection of Pneumocystis carinii in
       bronchoalveolar lavage specimens.
 DT    9505
 AU    Stager CE; Fraire AE; Kim HS; Estrada R; Davis JR; Greenberg D; Guy ES;
       Guntupalli KK; McBride RA; Department of Pathology, Baylor College of
       Medicine, Houston,; Tex.
 SO    Arch Pathol Lab Med. 1995 Feb;119(2):142-7. Unique Identifier : AIDSLINE
       MED/95150786
 AB    Four hundred forty-five bronchoalveolar lavage specimens from patients
       with the human immunodeficiency virus were preserved in Saccomano's
       fixative and stained for Pneumocystis carinii cysts by a modified method
       with Fungi-Fluor Solution A (Polysciences, Warrington, Pa) and the
       Genetic Systems Pneumocystis carinii Immunofluorescence Antibody
       (Genetic Systems Corp, Seattle, Wash). The majority of patients had been
       treated for suspected P carinii pneumonia for a few days prior to
       collection of bronchoalveolar lavage specimens. P carinii cysts were
       detected in 194 (43.6%) specimens. Both stains identified P carinii in
       166 (37.3%) specimens and were negative in 251 (56.4%), yielding a
       concordance rate of 93.7%. P carinii cysts were detected in 25 (5.6%)
       specimens by the Genetic Systems stain only, and in 3 (0.7%) specimens
       by the Fungi-Fluor stain only. The sensitivity for detecting cysts of P
       carinii was significantly greater with the Genetic Systems stain (P <
       .01).
 DE    Bronchoalveolar Lavage Fluid/*MICROBIOLOGY  Comparative Study
       *Fluorescent Antibody Technique  Human  HIV
       Infections/COMPLICATIONS/*MICROBIOLOGY  Pneumocystis carinii/*ISOLATION
       & PURIF  Pneumonia, Pneumocystis carinii/COMPLICATIONS/*MICROBIOLOGY
       Sensitivity and Specificity  Stains and Staining  Tissue
       Fixation/METHODS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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