Document 0259
 DOCN  M9550259
 TI    Screening for human immunodeficiency virus antibody in urine.
 DT    9505
 AU    Berrios DC; Avins AL; Haynes-Sanstad K; Eversley R; Woods WJ; Center for
       AIDS Prevention Studies, San Francisco, CA 94105.
 SO    Arch Pathol Lab Med. 1995 Feb;119(2):139-41. Unique Identifier :
       AIDSLINE MED/95150785
 AB    OBJECTIVE--To determine the diagnostic accuracy of an investigational
       test for human immunodeficiency virus (HIV) envelope antibodies in
       urine. DESIGN--Matched blood and urine specimens were tested for HIV by
       two independent laboratories, both of which were blinded to all results
       at the other site. Duplicate positive enzyme-linked immunoassay (EIA)
       results were confirmed by immunofluorescent antibody or western blot.
       SETTING--Six alcohol treatment centers in the San Francisco metropolitan
       area. PATIENTS--Five hundred ninety-two recovering alcoholics. MAIN
       OUTCOME MEASURES--Diagnosis of HIV infection by blood and urine EIA and
       western blot. RESULTS--The experimental urine EIA, when confirmed by
       urine western blot, led to a correct diagnosis in all samples. One
       sample was negative by urine EIA screening, positive by blood EIA, and
       exhibited an indeterminate blood western blot pattern (p24 band only).
       CONCLUSIONS--We encountered no false positive or false negative results
       using an investigational HIV antibody test for urine samples. There are
       several important advantages to HIV testing of urine versus serum or
       blood; however, there are also cogent reasons for limiting the use of
       alternative specimens for HIV testing.
 DE    Adolescence  Adult  Alcoholism/BLOOD/IMMUNOLOGY  Cross-Sectional Studies
       Enzyme-Linked Immunosorbent Assay  Feasibility Studies  Female  Human
       HIV Antibodies/BLOOD/*URINE  HIV-1/*IMMUNOLOGY  Male  Mass Screening
       Middle Age  Reproducibility of Results  Sensitivity and Specificity
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).